Fabrication of fieldeffect transistors (FET) immunosensors based on chemically modified graphenes for the ultrasensitive detection of lung cancer biomarkers

• Grant number: 16/04739-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2016
• End date: November 24, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Ana Carolina Mazarin de Moraes
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/22127-2 - Development of novel materials strategic for integrated analytical devices, AP.TEM
• Associated scholarship(s): 17/15882-0 - Solution-phase processing of two-dimensional nanomaterials for printed and flexible electronics, BE.EP.PD

Abstract

The early-stage diagnosis of cancer can be achieved through the detection and quantification of tumoral biomarkers. However, conventional immunoassays to detect these biomolecules are time-consuming, require large sample volumes, demand biomolecules labeling, etc. The field-effect transistors-based (FET) sensors offer enormous potential as alternative immunoassay tools because of higher sensitivity and selectivity, real-time response, miniaturization, and ease of integration in electronic chips. Graphene presents several advantages compared to other nanostructures such as remarkable electronic properties, large specific surface area, and biocompatibility. In this context, we propose in this project the large-scale fabrication of miniaturized and ultrasensitive FET immunosensors based on chemically modified graphenes for the label-free detection of the CYFRA 21-1 lung cancer biomarker. Graphene sheets will be produced by liquid exfoliation. This method is of cost-effective and high-throughput, and allows the deposition of the sheets on arbitrary substrates. The sensitivity and selectivity of microdevices can be improved by the combination of graphene with metallic nanoparticles, thereby forming graphene-nanoparticles hybrid materials. Therefore, we aim the increase of immunosensors sensibility by using graphene functionalized with platinum nanoparticles in the construction of microdevices. Specific antibodies will be immobilized on the graphene sensing channel to enable the biorecognition event with the antigen CYFRA 21-1, which will generate electrical signals. We will evaluate the ability of devices to detect and quantify very low concentrations of tumoral biomarkers. The development of these graphene-based devices impart their potential to become portable and commercial biosensing tools of low cost and high efficiency for the early diagnosis of serious diseases such as lung cancer.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, EVERSON T. S. G.; SOUTO, DENIO E. P.; BARRAGAN, JOSE T. C.; GIAROLA, JULIANA DE F.; DE MORAES, ANA C. M.; KUBOTA, LAURO T.. Electrochemical Biosensors in Point-of-Care Devices: Recent Advances and Future Trends. CHEMELECTROCHEM, v. 4, n. 4, p. 778-794, APR 2017. (13/22127-2, 16/04739-9, 15/13810-6)

MAZARIN DE MORAES, ANA CAROLINA; KUBOTA, LAURO TATSUO. Recent Trends in Field-Effect Transistors-Based Immunosensors. CHEMOSENSORS, v. 4, n. 4, DEC 2016. (13/22127-2, 16/04739-9)

BARRAGAN, JOSE T. C.; DA SILVA, EVERSON T. S. G.; DE MORAES, ANA C. M.; KUBOTA, LAURO T.. A novel approach for electroanalytical determinations employing discharge of pseudocapacitor by electroactive species. Analytica Chimica Acta, v. 1006, p. 1-9, MAY 2 2018. (14/50867-3, 13/22127-2, 16/04739-9, 15/13810-6)

BARRAGAN, JOSE T. C.; DA SILVA, EVERSON T. S. G.; DE MORAES, ANA C. M.; KUBOTA, LAURO T.. A novel approach for electroanalytical determinations employing discharge of pseudocapacitor by electroactive species (vol 1006, pg 1, 2018). Analytica Chimica Acta, v. 1031, p. 1-pg., 2018-11-15. (13/22127-2, 16/04739-9, 15/13810-6, 14/50867-3)