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Fabrication of fieldeffect transistors (FET) immunosensors based on chemically modified graphenes for the ultrasensitive detection of lung cancer biomarkers

Grant number: 16/04739-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2016
Effective date (End): November 24, 2018
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Lauro Tatsuo Kubota
Grantee:Ana Carolina Mazarin de Moraes
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/22127-2 - Development of novel materials strategic for integrated analytical devices, AP.TEM
Associated scholarship(s):17/15882-0 - Solution-phase processing of two-dimensional nanomaterials for printed and flexible electronics, BE.EP.PD


The early-stage diagnosis of cancer can be achieved through the detection and quantification of tumoral biomarkers. However, conventional immunoassays to detect these biomolecules are time-consuming, require large sample volumes, demand biomolecules labeling, etc. The field-effect transistors-based (FET) sensors offer enormous potential as alternative immunoassay tools because of higher sensitivity and selectivity, real-time response, miniaturization, and ease of integration in electronic chips. Graphene presents several advantages compared to other nanostructures such as remarkable electronic properties, large specific surface area, and biocompatibility. In this context, we propose in this project the large-scale fabrication of miniaturized and ultrasensitive FET immunosensors based on chemically modified graphenes for the label-free detection of the CYFRA 21-1 lung cancer biomarker. Graphene sheets will be produced by liquid exfoliation. This method is of cost-effective and high-throughput, and allows the deposition of the sheets on arbitrary substrates. The sensitivity and selectivity of microdevices can be improved by the combination of graphene with metallic nanoparticles, thereby forming graphene-nanoparticles hybrid materials. Therefore, we aim the increase of immunosensors sensibility by using graphene functionalized with platinum nanoparticles in the construction of microdevices. Specific antibodies will be immobilized on the graphene sensing channel to enable the biorecognition event with the antigen CYFRA 21-1, which will generate electrical signals. We will evaluate the ability of devices to detect and quantify very low concentrations of tumoral biomarkers. The development of these graphene-based devices impart their potential to become portable and commercial biosensing tools of low cost and high efficiency for the early diagnosis of serious diseases such as lung cancer.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARRAGAN, JOSE T. C.; DA SILVA, EVERSON T. S. G.; DE MORAES, ANA C. M.; KUBOTA, LAURO T. A novel approach for electroanalytical determinations employing discharge of pseudocapacitor by electroactive species. Analytica Chimica Acta, v. 1006, p. 1-9, MAY 2 2018. Web of Science Citations: 5.
DA SILVA, EVERSON T. S. G.; SOUTO, DENIO E. P.; BARRAGAN, JOSE T. C.; GIAROLA, JULIANA DE F.; DE MORAES, ANA C. M.; KUBOTA, LAURO T. Electrochemical Biosensors in Point-of-Care Devices: Recent Advances and Future Trends. CHEMELECTROCHEM, v. 4, n. 4, p. 778-794, APR 2017. Web of Science Citations: 57.
MAZARIN DE MORAES, ANA CAROLINA; KUBOTA, LAURO TATSUO. Recent Trends in Field-Effect Transistors-Based Immunosensors. CHEMOSENSORS, v. 4, n. 4 DEC 2016. Web of Science Citations: 20.

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