MALDI MS imaging investigation of the host response to visceral leishmaniasis

Autor(es):Mostrar menos -

Jaegger, C. F. ^[1] ; Negrao, F. ^[1] ; Assis, D. M. ^[2] ; Belaz, K. R. A. ^[1] ; Angolini, C. F. F. ^[1] ; Fernandes, A. M. A. P. ^[1] ; Santos, V. G. ^[1] ; Pimentel, A. ^[3] ; Abanades, D. R. ^[3] ; Giorgio, S. ^[3] ; Eberlin, M. N. ^[1] ; Rocha, D. F. O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[2] Bruker Daltonics Inc, Atibaia, SP - Brazil

^[3] Univ Campinas UNICAMP, Dept Anim Biol, Biol Inst, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOSYSTEMS; v. 13, n. 10, p. 1946-1953, OCT 2017.
• Citações Web of Science: 2

Resumo

Mass spectrometry imaging (MSI) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. Leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. Herein we have employed MALDI MSI to examine peptides and low molecular weight proteins (2 to 20 kDa) differentially expressed in the liver during visceral leishmaniasis in mice models. We analyzed liver sections of Balb/c mice infected with Leishmania infantum using the SCiLS Lab software for statistical analysis, which facilitated data interpretation and thus highlighted several key proteins and/or peptides. We proposed a decision tree classification for visceral leishmaniasis with distinct phases of the disease, which are named here as healthy, acute infection and chronic infection. Among others, the ion of m/z 4963 was the most important to identify acute infection and was tentatively identified as Thymosin beta 4. This peptide was previously established as a recovery factor in the human liver and might participate in the response of mice to Leishmania infection. This preliminary investigation shows the potential of MALDI MSI to complement classical compound selective imaging techniques and to explore new features not yet recognized by these approaches. (AU)

• Processo FAPESP: 16/11517-2 - Busca e identificação de candidatos a biomarcadores da leishmaniose cutânea através de espectrometria de massas
• Beneficiário: Fernanda Negrão Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/23767-0 - Pesquisa de biomarcadores e desenvolvimento de linhagens de Leishmania infantum
• Beneficiário: Selma Giorgio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/11100-6 - Fingerprinting de proteínas e lipídeos por MALDI-MS para diferenciação intra-espécie de isolados de Fusarium oxysporum f. sp. cubense
• Beneficiário: Daniele Fernanda de Oliveira Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/07206-0 - Busca de uma Assinatura Molecular da Receptividade em Bovinos por Espectrometria de Massas
• Beneficiário: Katia Roberta Anacleto Belaz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado