Lipid and protein fingerprinting for Fusarium oxysporum f. sp cubense strain-level classification

Autor(es):Mostrar menos -

Rocha, Daniele F. O. ^[1] ; Cunha, Cristiane M. S. ^[2] ; Belaz, Katia Roberta A. ^[1] ; dos Santos, Fabio N. ^[1] ; Hinz, Robert H. ^[3] ; Pereira, Adriana ^[4] ; Wicket, Ester ^[4] ; Andrade, Lidiane M. ^[5] ; Nascimento, Claudio A. O. ^[5] ; Visconti, Alexandre ^[4] ; Eberlin, Marcos N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, ThoMSon Mass Spectrometry Lab, Josue Castro St, BR-13083872 Sao Paulo - Brazil

^[2] Flora Biotecnol Ltd, Rua Silvio Ernesto da Silva 100, BR-88307751 Itajai, SC - Brazil

^[3] Empresa Sitio Barreiras Fruticultura Ltd, BR-63200 Missao Velha, Ceara - Brazil

^[4] Empresa Pesquisa Agr & Extenso Rural Santa Catari, Rodovia Antonio Heil 6-800, BR-88112318 Itajai, SC - Brazil

^[5] Univ Sao Paulo, Polytech Sch, Ave Prof Luciano Gualberto 380, BR-05508010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 409, n. 29, p. 6803-6812, NOV 2017.
• Citações Web of Science: 0

Resumo

Banana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability. (AU)

• Processo FAPESP: 13/11100-6 - Fingerprinting de proteínas e lipídeos por MALDI-MS para diferenciação intra-espécie de isolados de Fusarium oxysporum f. sp. cubense
• Beneficiário: Daniele Fernanda de Oliveira Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/07206-0 - Busca de uma Assinatura Molecular da Receptividade em Bovinos por Espectrometria de Massas
• Beneficiário: Katia Roberta Anacleto Belaz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado