A rabies virus vampire bat variant shows increased neuroinvasiveness in mice when compared to a carnivore variant

Autor(es):Mostrar menos -

Mesquita, Leonardo Pereira ^[1] ; Martins Gamon, Thais Helena ^[1] ; Campusano Cuevas, Silvia Elena ^[1] ; Asano, Karen Miyuki ^[2] ; Fahl, Willian de Oliveira ^[2] ; Iamamoto, Keila ^[2] ; Scheffer, Karin Correa ^[2] ; Achkar, Samira Maria ^[2] ; Zanatto, Dennis Albert ^[1] ; Cabrera Mori, Claudia Madalena ^[1] ; Maiorka, Paulo Cesar ^[1] ; Mori, Enio ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo, SP - Brazil

^[2] Inst Pasteur Sao Paulo, Ave Paulista 393, BR-01311000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 162, n. 12, p. 3671-3679, DEC 2017.
• Citações Web of Science: 1

Resumo

Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to evaluate the neuropathogenesis of infection with two Brazilian RABV street variants (variant 3 and crab-eating fox) in mice. BALB/c mice were inoculated with RABV through the footpad, with the 50% mouse lethal dose (LD50) determined by intracranial inoculation. The morbidity of rabies in mice infected with variant 3 and the crab-eating fox strain was 100% and 50%, respectively, with an incubation period of 7 and 6 days post-inoculation (dpi), respectively. The clinical disease in mice was similar with both strains, and it was characterized initially by weight loss, ruffled fur, hunched posture, and hind limb paralysis progressing to quadriplegia and recumbency at 9 to 12 dpi. Histological lesions within the central nervous system (CNS) characterized by nonsuppurative encephalomyelitis with neuronal degeneration and necrosis were observed in mice infected with variant 3 and those infected with the crab-eating fox variant. However, lesions and the presence of RABV antigen, were more widespread within the CNS of variant-3-infected mice, whereas in crab-eating fox-variant-infected mice, RABV antigens were more restricted to caudal areas of the CNS, such as the spinal cord and brainstem. In conclusion, the results shown here demonstrate that the RABV vampire bat strain (variant 3) has a higher potential for neuroinvasiveness than the carnivore variant. (AU)

• Processo FAPESP: 15/17807-0 - Estudo da neuroinvasividade e neurovirulência do vírus da raiva em amostras de sistema nervoso central de bovinos
• Beneficiário: Enio Mori
• Modalidade de apoio: Auxílio à Pesquisa - Regular