Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Autor(es)

Rossini, Eduardo Luiz ^[1] ; Milani, Maria Izabel ^[1] ; Carrilho, Emanuel ^[2] ; Pezza, Leonardo ^[1] ; Pezza, Helena Redigolo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio Mesquita Filho UNESP, Inst Quim, R Prof Francisco Degni 55, POB 355, BR-14801970 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim Sao Carlos, Ave Trabalhador Sao Carlense, 400, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 997, p. 16-23, JAN 2 2018.
• Citações Web of Science: 17

Resumo

In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (mu PAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffe reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective in-tensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L-1 and 50-600 mg L-1 for CRN and 16.5 mg L-1 and 50-500 mg L-1 for UA. There were no statistically significant differences between the results obtained using the mPAD and a chromatographic comparative method (Student's t-test at 95% confidence level). (c) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/21733-1 - Desenvolvimento de métodos analíticos de vanguarda utilizando spot test e dispositivo microfluidico em papel.
• Beneficiário: Helena Redigolo Pezza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/17749-7 - Determinação simultânea de biomarcadores de função renal em urina utilizando dispositivo analítico em papel microfluídico
• Beneficiário: Eduardo Luiz Rossini
• Modalidade de apoio: Bolsas no Brasil - Mestrado