Immunosensor for Pancreatic Cancer Based on Electrospun Nanofibers Coated with Carbon Nanotubes or Gold Nanoparticles

Autor(es)

Soares, Juliana C. ^[1] ; Iwaki, Leonardo E. O. ^{[2, 1]} ; Soares, Andrey C. ^{[2, 1]} ; Rodrigues, Valquiria C. ^[1] ; Melendez, Matias E. ^[3] ; Fregnani, Jose Humberto T. G. ^[3] ; Reis, Rui M. ^{[3, 4]} ; Carvalho, Andre L. ^[3] ; Correa, Daniel S. ^[5] ; Oliveira, Jr., Osvaldo N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-1356060 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mat Engn, BR-13563120 Sao Carlos, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil

^[4] Univ Minho, Life & Hlth Sci Res Inst ICVS, ICVS PT Govt Associate Lab 3Bs, P-4710057 Braga - Portugal

^[5] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS OMEGA; v. 2, n. 10, p. 6975-6983, OCT 2017.
• Citações Web of Science: 8

Resumo

We report the fabrication of immunosensors based on nanostructured mats of electrospun nanofibers of polyamide 6 and poly(allylamine hydrochloride) coated either with multiwalled carbon nanotubes (MWCNTs) or gold nanoparticles (AuNPs), whose three-dimensional structure was suitable for the immobilization of anti-CA19-9 antibodies to detect the pancreatic cancer biomarker CA19-9. Using impedance spectroscopy, the sensing platform was able to detect CA19-9 with a detection limit of 1.84 and 1.57 U mL(-1) for the nanostructured architectures containing MWCNTs and AuNPs, respectively. The high sensitivity achieved can be attributed to the irreversible adsorption between antibodies and antigens, as confirmed with polarization-modulated infrared reflection absorption spectroscopy. The adsorption mechanism was typical Langmuir-Freundlich processes. The high sensitivity and selectivity of the immunosensors were also explored in tests with blood serum from patients with distinct concentrations of CA19-9, for which the impedance spectra data were processed with a multidimensional projection technique. The robustness of the immunosensors in dealing with patient samples without suffering interference from analytes present in biological fluids is promising for a simple, effective diagnosis of pancreatic cancer at early stages. (AU)

• Processo FAPESP: 14/16789-5 - Materiais nanoestruturados híbridos baseados em polímeros conjugados e partículas metálicas aplicados em sensores
• Beneficiário: Daniel Souza Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático