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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immunosensor for Pancreatic Cancer Based on Electrospun Nanofibers Coated with Carbon Nanotubes or Gold Nanoparticles

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Autor(es):
Soares, Juliana C. [1] ; Iwaki, Leonardo E. O. [1, 2] ; Soares, Andrey C. [2, 1] ; Rodrigues, Valquiria C. [1] ; Melendez, Matias E. [3] ; Fregnani, Jose Humberto T. G. [3] ; Reis, Rui M. [3, 4] ; Carvalho, Andre L. [3] ; Correa, Daniel S. [5] ; Oliveira, Jr., Osvaldo N. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-1356060 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mat Engn, BR-13563120 Sao Carlos, SP - Brazil
[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil
[4] Univ Minho, Life & Hlth Sci Res Inst ICVS, ICVS PT Govt Associate Lab 3Bs, P-4710057 Braga - Portugal
[5] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: ACS OMEGA; v. 2, n. 10, p. 6975-6983, OCT 2017.
Citações Web of Science: 5
Resumo

We report the fabrication of immunosensors based on nanostructured mats of electrospun nanofibers of polyamide 6 and poly(allylamine hydrochloride) coated either with multiwalled carbon nanotubes (MWCNTs) or gold nanoparticles (AuNPs), whose three-dimensional structure was suitable for the immobilization of anti-CA19-9 antibodies to detect the pancreatic cancer biomarker CA19-9. Using impedance spectroscopy, the sensing platform was able to detect CA19-9 with a detection limit of 1.84 and 1.57 U mL(-1) for the nanostructured architectures containing MWCNTs and AuNPs, respectively. The high sensitivity achieved can be attributed to the irreversible adsorption between antibodies and antigens, as confirmed with polarization-modulated infrared reflection absorption spectroscopy. The adsorption mechanism was typical Langmuir-Freundlich processes. The high sensitivity and selectivity of the immunosensors were also explored in tests with blood serum from patients with distinct concentrations of CA19-9, for which the impedance spectra data were processed with a multidimensional projection technique. The robustness of the immunosensors in dealing with patient samples without suffering interference from analytes present in biological fluids is promising for a simple, effective diagnosis of pancreatic cancer at early stages. (AU)

Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/16789-5 - Materiais nanoestruturados híbridos baseados em polímeros conjugados e partículas metálicas aplicados em sensores
Beneficiário:Daniel Souza Corrêa
Linha de fomento: Auxílio à Pesquisa - Regular