Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The Effects of Photobiomodulation Delivered by Light-Emitting Diode on Stem Cells from Human Exfoliated Deciduous Teeth: A Study on the Relevance to Pluripotent Stem Cell Viability and Proliferation

Texto completo
Autor(es):
do Vale, Katia Llanos [1] ; Maria, Durvanei Augusto [2] ; Picoli, Lara Cristina [3] ; Deana, Alessandro Melo [1] ; Mascaro, Marcelo Betti [3] ; Mesquita Ferrari, Raquel Agnelli [4, 1] ; Bussadori, Sandra Kalil [4, 1] ; Santos Fernandes, Kristianne Porta [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Nove de Julho UNINOVE, Biophoton Appl Hlth Sci Post Graduat Program, Rua Vergueiro 249 Liberdade, BR-01504001 Sao Paulo, SP - Brazil
[2] Butantan Inst, Biochem & Biophys Lab, Sao Paulo - Brazil
[3] Nove de Julho Univ UNINOVE, Sch Dent, Sao Paulo - Brazil
[4] Univ Nove de Julho UNINOVE, Rehabil Sci Post Grad Program, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Photomedicine and Laser Surgery; v. 35, n. 12, p. 659-665, DEC 2017.
Citações Web of Science: 0
Resumo

Objective: Photobiomodulation (PBM) can modulate the proliferation of some types of stem cells. However, few reports have addressed the effects of PBM delivered by light-emitting diode (LED) on stem cells obtained from the pulp tissue of deciduous teeth. The aim of the present study was to investigate the effect of PBM delivered by red LED (630nm, 75mW, 37mW/cm(2)) with different radiant exposures on the cell cycle, mitochondrial membrane potential, and senescence of stem cells from human exfoliated deciduous teeth (SHED). Materials and methods: Cultures were irradiated with LED (2, 4, 8, 16, and 32J/cm(2)). After 24h, the cell cycle and mitochondrial membrane potential of the cultures were evaluated using flow cytometry. Nonirradiated cultures served as control. Results: Cultures irradiated with 16J/cm(2) had higher percentages of cells in the synthesis phase than control cultures (p<0.05), and no significant differences were found regarding the percentage of cells with viable mitochondria between irradiated and control cultures. No significant difference in cell senescence was found between control cultures and cultures irradiated with 2 or 16J/cm(2). Conclusions: LED irradiation at 630nm (37mW/cm(2), 75mW) with radiant exposure of 16J/cm(2) was capable of inducing a proliferative response in stem cells from the pulp tissue of deciduous teeth without affecting mitochondrial function or inducing senescence. (AU)

Processo FAPESP: 13/07502-1 - Efeito da laserterapia sobre a expressão gênica e a produção de citocinas, quimiocinas e fatores de crescimento envolvidos no reparo muscular
Beneficiário:Kristianne Porta Santos Fernandes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/12381-1 - Análise comparativa do efeito da laserterapia prévia associada ou não ao tratamento pós sobre a modulação de citocinas inflamatórias, do estresse oxidativo e a determinação do tipo de fibra muscular após lesão aguda
Beneficiário:Raquel Agnelli Mesquita Ferrari
Linha de fomento: Auxílio à Pesquisa - Regular