Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

Autor(es)

Perlatti D'Alpino, Paulo Henrique ^{[1, 2]} ; Diniz de Dantas Moura, Gioconda Emanuella ^[3] ; de Arruda Barbosa, Silvana Coelho ^[1] ; Marques, Lygia de Azevedo ^[4] ; Eberlin, Marcos Nogueira ^[4] ; Nascimento, Fabio Dupart ^[5] ; dos Santos Tersariol, Ivarne Luis ^{[3, 5]}

Afiliação do(s) autor(es)

^[1] Univ Anhanguera Sao Paulo UNIAN SP, Biotechnol & Innovat Hlth Program, Sao Paulo, SP - Brazil

^[2] Univ Anhanguera Sao Paulo UNIAN SP, Biomat Dent Program, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Biochem, Mol Biol Div, Sao Paulo - Brazil

^[4] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[5] Univ Mogi Das Cruzes, Interdisciplinary Ctr Biochem Invest CIIB, Mogi Das Cruzes, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 33, n. 12, p. 1402-1415, DEC 2017.
• Citações Web of Science: 5

Resumo

Objectives. To evaluate the cytotoxic effects of exposing odontoblast cells to a variety of commercial self-adhesive cements polymerized using different activation modes. Methods. Five cements: MaxCem Elite (MAX), Bifix SE (BSE), G-Cem LinkAce (GCE), Clearfil SA Luting (CAS), and RelyX U200 (U200) were mixed, dispensed into molds, and distributed in groups, according to polymerization protocols: immediate photoactivation; delayed photoactivation (10 min self-curing plus light-activation); and chemical activation (no light exposure). Immortalized rat odontoblast cells (MDPC-23) were cultured. Cell viability was assessed by Trypan Blue staining and total cell death was assessed by annexin V-APC/7-AAD double staining and flow cytometry. Volatilized compounds from polymerized specimens of cements were evaluated by gas chromatography/mass spectrometry (GC-MS). Data was analyzed with 2-way ANOVA/Tukey tests (alpha = 0.05). Results. Exposure to all of the cements tested significantly reduced the cell viability, irrespective of the activation protocol (p < 0.05). The least harmful cements were CSA and U200. Total death of cells significantly increased when exposed to BSE, GCE, and MAX, especially when chemically activated (p < 0.05). Characteristic apoptotic cells increased after exposure to cements, mainly for MAX, regardless of the activation mode. Chemical activation of MAX also induced necrosis. Moreover, GCE and MAX exhibited higher percentages of late apoptotic/dead cells. Chromatograms revealed 28 compounds released from the cements tested, some of them with known carcinogenic effects. Selection of self-adhesive cements and polymerization protocols affect the cytotoxicity and cell viability of odontoblastic cells. Clinical significance. Despite the simplified cementation protocol, care is needed when cementing indirect restorations with self-adhesive cements, especially on recently exposed dentin. This category of material may cause differential cytotoxic effects and should be considered when selecting a cement. This is particularly true in clinical cases of light attenuation, where the polymerization depends on chemical activation, inducing higher cytotoxic damages when using some of the cements tested. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/05822-9 - Receptores ativados por proteases (PARs) no complexo dentina-polpa: identificação, modulação e sinalização celular durante o desenvolvimento da doença cárie
• Beneficiário: Fábio Dupart Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores