Cellulase Production by Trichosporon laibachii

Autor(es)

Giese, Ellen C. ^{[1, 2]} ; Dussan, Kelly J. ^{[1, 3]} ; Pierozzi, Mauricio ^[1] ; Chandel, Anuj K. ^[1] ; Pagnocca, Fernando C. ^[4] ; da Silva, Silvio S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Engn Lorena, Dept Biotechnol, BR-12602810 Lorena, SP - Brazil

^[2] Ctr Mineral Technol, BR-21941908 Rio De Janeiro, RJ - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Chem Technol, BR-14800900 Araraquara, SP - Brazil

^[4] UNESP, Biosci Inst Rio Claro, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORBITAL-THE ELECTRONIC JOURNAL OF CHEMISTRY; v. 9, n. 4, p. 271-278, JUL-SEP 2017.
• Citações Web of Science: 1

Resumo

The ability of twenty-three yeast strains isolated from decayed wood and the fungus garden of attine ants to produce hemi-and cellulolytic hydrolases in a chemically defined medium was acessed. Xylanase, beta-glucosidase and cellulase activities were found in yeasts strains in the genera of Cryptococcus, Trichosporon, Debaryomyces and Pichia. Among the isolated microorganisms, two strains of Trichosporon laibachii MG270406-1A14 strain showed higher cellulase titers. Several biochemical and physiological parameters were investigated for optimum cellulase production under submerged fermentation. Enzyme induction was also examined using different carbon and nitrogen sources. Cellobiose and ammonium sulphate were found best carbon (C) and nitrogen (N) sources to enhance carboxymethylcellulase production. A 22-factorial design with center points was developed to optimize enzyme production, and data analysed by the response surface method. Maximal enzyme titres (0.3 U mL(-1)) occurred at initial pH 6.0, C/N ratio 9.0 and 5 days. (AU)

• Processo FAPESP: 08/57926-4 - Etanol: pesquisa e desenvolvimento visando ao aproveitamento integrado do bagaço de cana-de-açúcar para a produção biotecnológica do etanol lignocelulósico
• Beneficiário: Silvio Silvério da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático