Agricultural Feedstock Supplemented with Manganese for Biosurfactant Production by Bacillus subtilis

Autor(es)

Cruz, Jaqueline Matos ^[1] ; Hughes, Cian ^[2] ; Quilty, Brid ^[3] ; Montagnolli, Renato Nallin ^[1] ; Bidoia, Ederio Dino ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biochem & Microbiol, Av 24-A, 1515, BR-13506900 Rio Claro - Brazil

^[2] Dublin City Univ, Sch Engn, Dublin 9 - Ireland

^[3] Dublin City Univ, Sch Biotechnol, Dublin 9 - Ireland

• Tipo de documento: Artigo Científico
• Fonte: WASTE AND BIOMASS VALORIZATION; v. 9, n. 4, p. 613-618, APR 2018.
• Citações Web of Science: 2

Resumo

The greatest challenge in producing biomolecules at industrial scale is cost. In order to provide cheaper sources, the present study describes the production of biosurfactant using a low-cost medium supplemented with manganese. The feedstock used to produce biosurfactant was crude glycerol, a by-product of biodiesel production. Results showed that 5% (v/v) of glycerol and 0.05 mM of manganese was the best combination to produce biosurfactant. The produced biosurfactant was able to reduce surface tension and showed emulsification activity in diesel fuel. The main functional groups of the biosurfactant were identified by H-1 NMR and FTIR spectra. We identified the molecule as surfactin based on comparison with surfactin standard spectra described in the literature. This study showed conversion of low-value glycerol into value-added products as biosurfactant. The use of a by-product as a carbon source for biosurfactant production is a possible strategy for reducing production costs. In addition, biosurfactant production by Bacillus subtilis can be considered safe and commercially viable, because it is a non-pathogenic bacterium. (AU)

• Processo FAPESP: 13/13813-0 - Utilização do glicerol para produção de biossurfactante por Bacillus subtilis e como co- substrato na biodegradação de diesel e biodiesel em solo
• Beneficiário: Jaqueline Matos Cruz
• Modalidade de apoio: Bolsas no Brasil - Doutorado