Effect of methylene blue-mediated antimicrobial photodynamic therapy on dentin caries microcosms

Autor(es)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Gutierrez, Eliezer ^[1] ; Dionisio, Evandro Jose ^[1] ; Oliveira, Thais Marchini ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Rios, Daniela ^[1] ; Aparecida Andrade Moreira Machado, Maria ^[1] ; Cruvinel, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 33, n. 3, p. 479-487, APR 2018.
• Citações Web of Science: 7

Resumo

Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjuvant treatment of dental caries, although there are no well-defined protocols to its clinical application. This study aimed to evaluate the influence of aPDT on the viability of microorganisms, vitality of biofilms, and lactic acid production of dentin caries microcosms. Biofilms were grown on bovine dentin discs in anaerobic conditions at 37 A degrees C for 5 days, inoculating infected carious dentin in modified McBain medium plus 1% sucrose. The biofilms were treated by the combination of deionized water or 100 mg L-1 methylene blue (MB) with 0, 37.5, or 75 J cm(-2) LED at 630 nm. The counts of total microorganisms, total streptococci, mutans streptococci, and total lactobacilli were determined by colony-forming units (CFU). The vitality of microbial cells in intact biofilms was measured by confocal laser scanning microscope (CLSM). The lactic acid production was analyzed by enzymatic spectrophotometry at 340 nm. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn's tests (P < 0.05). MB and 37.5 J cm(-2) LED alone did not interfere in the viability of microorganisms, unlike 75 J cm(-2) LED alone that decreased the total microorganism and lactobacillus counts. The combination of MB and 75 J cm(-2) LED reduced the viability of all microorganisms and the vitality of intact biofilms. The production of lactic acid was statistically lower in all treatment groups in comparison with that of the control (no treatment), except for MB alone. Therefore, the MB-mediated aPDT was effective in controlling the viability, vitality and the acidogenicity of dentin caries microcosms. (AU)

• Processo FAPESP: 15/08595-9 - Efeito da terapia fotodinâmica antimicrobiana sobre microrganismos específicos provenientes de microcosmos de lesões de cárie em dentina
• Beneficiário: Daniela Alejandra Cusicanqui Méndez
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/10897-0 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Thiago Cruvinel da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22238-1 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Eliézer Gutierres
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica