Repeated Domperidone treatment modulates pulmonary cytokines in LPS-induced acute lung injury in mice

Autor(es)

Barreto, Talita Rossetto ^[1] ; Costola-de-Souza, Carolina ^[1] ; Margatho, Rafael Oliveira ^[1] ; Queiroz-Hazarbassanov, Nicolle ^[1] ; Rodrigues, Sandra Campos ^[1] ; Felicio, Luciano Freitas ^[1] ; Palermo-Neto, Joao ^[1] ; Zager, Adriano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Neuroimmunomodulat Res Grp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 56, p. 43-50, MAR 2018.
• Citações Web of Science: 6

Resumo

The dopaminergic antagonist drug Domperidone has immunomodulatory effects. We investigated the effects of repeated Domperidone treatment in a model of Lypopolyssacharide (LPS)-induced acute lung inflammation. Adult C57BL/6J mice were treated with either Vehicle or Domperidone for 5 days, and challenged intranasally with LPS in the following day. The behavior of mice was analyzed in the open field and elevated plus-maze test before and 24 h after LPS challenge. The bronchoalveolar lavage fluid, blood and lung tissue were collected 24 h and 48 h after LPS challenge. Domperidone treatment increased LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 production in the bronchoalveolar lavage fluid, without altering tissue damage and the number of immune cells in the lungs and circulation. Locomotor and anxiety-like behavior were unchanged after Domperidone and/or LPS treatment. Cytokine data indicate that Domperidone promotes a change in activity of other cell types, likely alveolar epithelial cells, without affecting immune cell migration in the present model. Due to the role of these cytokines in progression of inflammation, Domperidone treatment may exacerbate a subsequent inflammatory injury. (AU)

• Processo FAPESP: 15/02441-0 - Efeitos da hiperprolactinemia em um modelo murino de inflamação pulmonar aguda
• Beneficiário: Talita Rossetto Barreto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/22364-2 - Hiperprolactinemia e inflamação alérgica pulmonar
• Beneficiário: Luciano Freitas Felicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18921-5 - Avaliação do efeito do modafinil sobre a imunidade, neuroinflamação e desenvolvimento da encefalomielite autoimune experimental em camundongos
• Beneficiário: Adriano Zager
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático