Comparison of the production of enzymes to cell wall hydrolysis using different carbon sources by Penicillium echinulaturn strains and its hydrolysis potential for lignocelullosic biomass

Autor(es)

Hahn Schneider, Willian Daniel ^[1] ; Goncalves, Thiago Augusto ^[2] ; Uchima, Cristiane Akemi ^[3] ; dos Reis, Laisa ^[1] ; Fontana, Roselei Claudete ^[1] ; Squina, Fabio Marcio ^[2] ; Pinheiro Dillon, Aldo Jose ^[1] ; Camassola, Marli ^[1]

Afiliação do(s) autor(es)

^[1] Univ Caxias Do Sul, Inst Biotechnol, Enzymes & Biomass Lab, Rua Francisco Getulio Vargas 1130, BR-95070560 Caxias Do Sul, RS - Brazil

^[2] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Km 92-5, BR-18023000 Sorocaba, SP - Brazil

^[3] CTBE, Brazilian Bioethanol Sci & Technol Lab, Rua Giuseppe Maximo Scolfaro 10000, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 66, p. 162-170, MAR 2018.
• Citações Web of Science: 2

Resumo

One of the current challenges to produce second-generation ethanol is to obtain an efficient enzyme complex for hydrolysis of lignocellulosic biomass. Genetic improvement of lignocellulolytic fungi has helped to obtain strains that secrete higher enzymatic titers. This article analyzes the enzymatic profile of two strains of Penicillium echinulattun, wild type 2HH and mutant S1M29, grown on different carbon sources (sugarcane bagasse pre-treated by steam explosion, cellulose, glucose, and glycerol) at different cultivation times. To determine enzyme activities, 23 substrates were used. P. echinulaturn showed potential to produce cellulases, hemicellulases, esterases, and in minor proportions, pectinases. The mutant strain showed the highest enzymatic titers to most enzymes considered and its enzymatic broth was used in the enzymatic hydrolysis of vegetable biomass. This study broadened the understanding of the enzyme complex of this fungus and the importance of future studies with the mutant strain for use in the production of second-generation ethanol. (AU)

• Processo FAPESP: 08/58037-9 - Geração de biblioteca para conversão enzimática de biomassa a partir de metagenoma do solo
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores