Urea impedimetric biosensing using electrospun nanofibers modified with zinc oxide nanoparticles

Autor(es)

Migliorini, Fernanda L. ^[1] ; Sanfelice, Rafaela C. ^[2] ; Mercante, Luiza A. ^{[3, 1]} ; Andre, Rafaela S. ^[1] ; Mattoso, Luiz H. C. ^{[3, 1]} ; Correa, Daniel S. ^[1]

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Triangulo Mineiro, ICTE, Dept Engn Quim, Uberaba, MG - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Mat Engn, PPG CEM, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Surface Science; v. 443, p. 18-23, JUN 15 2018.
• Citações Web of Science: 6

Resumo

Reliable analytical techniques to evaluate dairy products, including milk, are of outmost importance to ensure food safety against contaminants. Among possible substances employed as adulterants in milk, urea raises deep concern due to its harmful effects to consumer's health. In the present study, a biosensing platform was developed to be applied in the electrochemical detection of urea. The sensing platform was fabricated using polymeric electrospun nanofibers of polyamide 6 (PA6) and polypyrrole (PPy) deposited onto fluorine doped tin oxide (FTO) electrodes, which were then modified with zinc oxide nanoparticles (ZnO). This material showed excellent properties for the immobilization of urease enzyme, conferring the FTO/PA6/PPy/ZnO/urease electrode high sensitivity for urea detection within the concentration range between 0.1 and 250 mg dL(-1) with a limit of detection of 0.011 mg dL(-1). The results achieved evidence the potential of electrospun nanofibers-based electrodes for applications in biosensors aiming at dairy products analysis. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/23793-4 - Desenvolvimento de sensores nanoestruturados visando a detecção de compostos voláteis indicadores da qualidade de alimentos
• Beneficiário: Rafaela da Silveira Andre
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/16789-5 - Materiais nanoestruturados híbridos baseados em polímeros conjugados e partículas metálicas aplicados em sensores
• Beneficiário: Daniel Souza Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular