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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Biochemical and biophysical characterization of novel GH10 xylanase prospected from a sugar cane bagasse compost-derived microbial consortia

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Autor(es):
Evangelista, Danilo Elton [1] ; Seiki Kadowaki, Marco Antonio [1] ; Mello, Bruno Luan [1] ; Polikarpov, Igor [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 109, p. 560-568, APR 2018.
Citações Web of Science: 4
Resumo

Environmental issues are promoting the development of innovative technologies for the production of renewable energy and ``green products{''} from plant biomass residues. These technologies rely on the conversion of the plant cell wall (PCW) polysaccharides into simple sugars, which involve synergistic activities of different PCW degrading enzymes, including xylanases; these are widely applied in food and feed sectors, paper and textile industries, among others. We cloned, expressed and biochemically characterized a novel xylanase (Xyn10) from the GH10 identified in a metatranscriptome of compost derived microbial consortia and determined its low-resolution SAXS molecular envelope in solution. Our results reveal that Xyn I 0 is a monomeric flexible globular enzyme, with high stability with a broad pH range from 4 to 10 and optimal activity conditions at pH 7 and 40 degrees C. Only 10% of activity loss was observed after the enzyme was incubated for 30 h at 40 degrees C with a pH ranging from 5 to 10. Moreover, Xyn10 maintained 100% of its initial activity after incubation for 120 h at 40 degrees C and 51% after incubation for 24 h at 50 degrees C (pH = 7.0). Xyn10 shows endocatalytic activity towards xylan and arabinoxylan, liberating xylose, xylobiose, 1,2-alpha-D-methylglucuronic acid decorated xylotriose, and 1,3-alpha-L-arabinofuranose decorated xylobiose and xylotriose oligosaccharides. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 15/13684-0 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/20505-4 - Duas classes importantes de glicosil hidrolases: estudos funcionais e análise estrutural
Beneficiário:Marco Antonio Seiki Kadowaki
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/21608-1 - Identificação e caracterização de novas enzimas com potencial na digestão de biomassa lignocelulósica
Beneficiário:Bruno Luan Soares Paula de Mello
Linha de fomento: Bolsas no Brasil - Doutorado Direto