Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Autor(es)

Borges, Aline Chiodi ^{[1, 2]} ; Castaldelli Nishime, Thalita Mayumi ^[3] ; Kostov, Konstantin Georgiev ^[3] ; Gouvea Lima, Gabriela de Morais ^{[1, 2]} ; Lacerda Gontijo, Aline Vidal ^{[1, 2, 4]} ; Martins Marchesotti de Carvalho, Juliana Nobrega ^{[1, 2]} ; Honda, Roberto Yzumi ^[3] ; Koga-Ito, Cristiane Yumi ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Environm Engn, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Oral Biopathol Grad Program, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Chem & Phys, Fac Engn, Guaratingueta, SP - Brazil

^[4] Univ Estadual Campinas, Dept Biosci & Technol Bioact Prod, Inst Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL PLASMA MEDICINE; v. 7-8, p. 9-15, DEC 2017.
• Citações Web of Science: 2

Resumo

The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity and genotoxicity of He-CAPPJ to Vero cells. The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay and genotoxicity was investigated by micronucleus test. A promising effect of He-CAPPJ on morphogenesis was observed, with a reduction of almost 40 times in the filamentation rate compared to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. In conclusion, He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity after short treatment times. Periods of exposure effective against biofilms were cytotoxic to Vero cells. He-CAPPJ did not show genotoxic effect. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections. (AU)

• Processo FAPESP: 15/03470-3 - Aplicações do plasma de baixa temperatura sob pressão atmosférica na odontologia
• Beneficiário: Gabriela de Morais Gouvêa Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/07196-6 - Prospecção de alternativas terapêuticas inovadoras para doenças infecciosas de interesse odontológico: jato de plasma frio sob pressão atmosférica e nanoencapsulado de substância bioativa
• Beneficiário: Cristiane Yumi Koga Ito
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02354-7 - Atividade antifúngica e genotoxicidade do jato de plasma frio sob pressão atmosférica
• Beneficiário: Aline Chiodi Borges
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/21989-6 - Desenvolvimento de Jatos de Plasma Frio em Pressão Atmosférica para Tratamento de Materiais, Deposição de Filmes Finos e Aplicações Biomédicas
• Beneficiário: Konstantin Georgiev Kostov
• Modalidade de apoio: Auxílio à Pesquisa - Regular