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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Full text
Author(s):
Borges, Aline Chiodi [1, 2] ; Castaldelli Nishime, Thalita Mayumi [3] ; Kostov, Konstantin Georgiev [3] ; Gouvea Lima, Gabriela de Morais [1, 2] ; Lacerda Gontijo, Aline Vidal [1, 2, 4] ; Martins Marchesotti de Carvalho, Juliana Nobrega [1, 2] ; Honda, Roberto Yzumi [3] ; Koga-Ito, Cristiane Yumi [1, 2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Environm Engn, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil
[2] Sao Paulo State Univ UNESP, Oral Biopathol Grad Program, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Chem & Phys, Fac Engn, Guaratingueta, SP - Brazil
[4] Univ Estadual Campinas, Dept Biosci & Technol Bioact Prod, Inst Biol, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CLINICAL PLASMA MEDICINE; v. 7-8, p. 9-15, DEC 2017.
Web of Science Citations: 2
Abstract

The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity and genotoxicity of He-CAPPJ to Vero cells. The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay and genotoxicity was investigated by micronucleus test. A promising effect of He-CAPPJ on morphogenesis was observed, with a reduction of almost 40 times in the filamentation rate compared to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. In conclusion, He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity after short treatment times. Periods of exposure effective against biofilms were cytotoxic to Vero cells. He-CAPPJ did not show genotoxic effect. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections. (AU)

FAPESP's process: 15/03470-3 - Aplications of low temperature atmospheric pressure plasma in dentistry
Grantee:Gabriela de Morais Gouvêa Lima
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance
Grantee:Cristiane Yumi Koga Ito
Support type: Regular Research Grants
FAPESP's process: 15/21989-6 - Development of Cold Atmospheric Pressure Plasma Jets for Material Treatment, Thin Films Deposition and Biomedical Applications
Grantee:Konstantin Georgiev Kostov
Support type: Regular Research Grants
FAPESP's process: 14/02354-7 - Antifungal activity and genotoxicity of atmospheric pressure nonthermal plasma jet
Grantee:Aline Chiodi Borges
Support type: Scholarships in Brazil - Doctorate