Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development

Autor(es)

Ricci, Juliana M. B. ^[1] ; Martinez, Emanuel R. M. ^[1] ; Butzge, Arno J. ^[1] ; Doretto, Lucas B. ^[1] ; Oliveira, Marcos A. ^[1] ; Bombardelli, Robie Allan ^[2] ; Bogerd, Jan ^[3] ; Nobrega, Rafael H. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil

^[2] Univ Estadual Oeste Parana, Ctr Engn & Exact Sci, Rua Fac 645, BR-85903000 Toledo, PR - Brazil

^[3] Univ Utrecht, Div Dev Biol, Dept Biol, Reprod Biol Grp, Fac Sci, Hugo R Kruyt Bldg, Padualaan 8, NL-3584 CH Utrecht - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 654, p. 116-126, MAY 15 2018.
• Citações Web of Science: 3

Resumo

We have characterized the full-length vasa cDNA from Jundia, Rhamdia quelen (Heptapteridae, Siluriformes). vasa encodes a member of the DEAD-box protein family of ATP-dependent RNA helicases. This protein is highly conserved among different organisms and its role is associated with RNA metabolism. In the majority of the investigated species, vasa is restricted to the germ cell lineage and its expression has been used to study germline development in many organisms, including fish. The deduced R. quelen vasa amino acid sequence displayed high similarity with Vasa protein sequences from other organisms, and did not cluster with PL10 or P68 DEAD-box protein subfamilies. We also reported that there is no other isoform for vasa mRNA in R. quelen gonads. Expression analysis by RT-PCR and qPCR showed vasa transcripts exclusively expressed in the germ cells of R. quelen gonads. R. quelen vasa mRNA was maternally inherited, and was detected in the migrating primordial germ cells (PGCs) until 264 h post-fertilization during embryonic and larval development. This work has characterized for the first time the full-length R. quelen vasa cDNA, and describes its expression patterns during R. quelen embryonic and larval development. Our results will contribute to the basic reproductive biology of this native species, and will support studies using vasa as a germ cell marker in different biotechnological studies, such as germ cell transplantation. (AU)

• Processo FAPESP: 12/00423-6 - Regulação parácrina e molecular do nicho de espermatogônias tronco em zebrafish (Danio rerio) e uso da carpa comum (Cyprinus carpio) como modelo para atrasar a diferenciação espermatogonial durante a puberdade
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07620-7 - Banco de espermatogônias tronco em peixes para conservação do genótipo de espécies ameaçadas e de interesse econômico
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores