Isoflavonoids from Brazilian red propolis down-regulate the expression of cancer-related target proteins: A pharmacogenomic analysis

Autor(es)

Nani, Bruno Dias ^[1] ; Franchin, Marcelo ^[1] ; Lazarini, Josy Goldoni ^[1] ; Freires, Irlan Almeida ^[2] ; da Cunha, Marcos Guilherme ^[3] ; Bueno-Silva, Bruno ^[1] ; de Alencar, Severino Matias ^[4] ; Murata, Ramiro Mendonca ^{[5, 6]} ; Rosalen, Pedro Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[2] Univ Florida, Coll Dent, Dept Oral Biol, 1395 Ctr Dr, Gainesville, FL 32610 - USA

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Agri Food Ind Food & Nutr Luiz de Queiroz, Coll Agr, Piracicaba, SP - Brazil

^[5] East Carolina Univ, Sch Dent Med, Dept Fdn Sci, Greenville, NC 27858 - USA

^[6] East Carolina Univ, Brody Sch Med, Dept Microbiol & Immunol, Greenville, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: Phytotherapy Research; v. 32, n. 4, p. 750-754, APR 2018.
• Citações Web of Science: 2

Resumo

Vestitol and neovestitol are bioactive isoflavonoids isolated from Brazilian red propolis, a unique Apis melifera type of propolis botanically originated from Dalbergia ecastophyllum. Although these molecules have relevant biological effects, including anticancer and immunomodulatory activities, their mechanism(s) of action and the affected pathways remain largely unknown. Here, we carried out a pharmacogenomic analysis to investigate the effects of vestitol and neovestitol on the whole-genome expression in human tumor cells, particularly cancer-related target proteins. HeLa cells were exposed to the compounds at IC20 and genomic information of treated cells was analyzed using the Illumina transcriptome system and GeneGo MetaCore software. Our results showed that vestitol (IC20=214.7M) reduced the expression of genes enrolled with the alpha tubulin (fold -3.7), tubulin in microtubules (fold -3.7), and histone h3 (fold=-3.03), and that treatment with neovestitol (IC20=102.91M) downregulated prostaglandin E synthase gene (fold=-3.12), which are considered ideal targets for anticancer therapy. These data open avenues for the study of vestitol and neovestitol as potential promising candidates for anticancer therapy. Toxicological, non-clinical, and clinical validation of the findings presented herein is needed. (AU)

• Processo FAPESP: 12/22378-2 - Estudo in vivo e in vitro da atividade de compostos isolados de própolis brasileiras sobre a modulação da migração de neutrófilos no processo inflamatório
• Beneficiário: Pedro Luiz Rosalen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23635-6 - Avaliação farmacológica e isolamento de moléculas bioativas da fração hexânica da geoprópolis de melipona scutellaris
• Beneficiário: Marcos Guilherme da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado