Isoflavonoids from Brazilian red propolis down-regulate the expression of cancer-related target proteins: A pharmacogenomic analysis

Author(s)

Nani, Bruno Dias ^[1] ; Franchin, Marcelo ^[1] ; Lazarini, Josy Goldoni ^[1] ; Freires, Irlan Almeida ^[2] ; da Cunha, Marcos Guilherme ^[3] ; Bueno-Silva, Bruno ^[1] ; de Alencar, Severino Matias ^[4] ; Murata, Ramiro Mendonca ^{[5, 6]} ; Rosalen, Pedro Luiz ^[1]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[2] Univ Florida, Coll Dent, Dept Oral Biol, 1395 Ctr Dr, Gainesville, FL 32610 - USA

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Agri Food Ind Food & Nutr Luiz de Queiroz, Coll Agr, Piracicaba, SP - Brazil

^[5] East Carolina Univ, Sch Dent Med, Dept Fdn Sci, Greenville, NC 27858 - USA

^[6] East Carolina Univ, Brody Sch Med, Dept Microbiol & Immunol, Greenville, NC - USA

• Document type: Journal article
• Source: Phytotherapy Research; v. 32, n. 4, p. 750-754, APR 2018.
• Web of Science Citations: 2

Abstract

Vestitol and neovestitol are bioactive isoflavonoids isolated from Brazilian red propolis, a unique Apis melifera type of propolis botanically originated from Dalbergia ecastophyllum. Although these molecules have relevant biological effects, including anticancer and immunomodulatory activities, their mechanism(s) of action and the affected pathways remain largely unknown. Here, we carried out a pharmacogenomic analysis to investigate the effects of vestitol and neovestitol on the whole-genome expression in human tumor cells, particularly cancer-related target proteins. HeLa cells were exposed to the compounds at IC20 and genomic information of treated cells was analyzed using the Illumina transcriptome system and GeneGo MetaCore software. Our results showed that vestitol (IC20=214.7M) reduced the expression of genes enrolled with the alpha tubulin (fold -3.7), tubulin in microtubules (fold -3.7), and histone h3 (fold=-3.03), and that treatment with neovestitol (IC20=102.91M) downregulated prostaglandin E synthase gene (fold=-3.12), which are considered ideal targets for anticancer therapy. These data open avenues for the study of vestitol and neovestitol as potential promising candidates for anticancer therapy. Toxicological, non-clinical, and clinical validation of the findings presented herein is needed. (AU)

• FAPESP's process: 12/22378-2 - In vivo and in vitro study of activity of isolated compounds from Brazilian propolis on modulation of neutrophils migration in the inflammatory process
• Grantee: Pedro Luiz Rosalen
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23635-6 - Isolation and pharmacological evaluation of bioactive molecules from hexane fraction of M. scutellaris geopropolis
• Grantee: Marcos Guilherme da Cunha
• Support Opportunities: Scholarships in Brazil - Doctorate