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Isolation and pharmacological evaluation of bioactive molecules from hexane fraction of M. scutellaris geopropolis

Grant number: 11/23635-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Pedro Luiz Rosalen
Grantee:Marcos Guilherme da Cunha
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):12/22002-2 - Chemical elucidation of biocompounds and molecular targets of geopropolis on cancer cell lines, BE.EP.DR


Natural products are historically considered as sources of bioactive compounds against a variety of pathological conditions. Among these products, propolis, a resinous substance collected by bees, stands out for presenting a considerable range of pharmacological activities related. Its chemical composition is variable, depending on the biodiversity of the region where it is collected, and may have different patterns of biological responses. However, most studies have reported the properties inherent in propolis collected by Apis mellifera bees, whereas other types of propolis collected by bees from different species continue to edge of knowledge and lack of information on its chemical constitution and biological properties. The geopropolis is a different type of propolis collected by native stingless bees, for example, Melipona scutellaris. In addition to resins, this product also contains wax and soil in its composition, making it unique and also devoid of economic value added, with few studies on their chemical and biological activities. Recent studies indicate that this has essentially apolar chemical composition, and its chemical hexane fraction has interesting antimicrobial activity against Staphylococcus aureus, anti-inflammatory and analgesic, but its potential, while promising, is still not fully elucidated. The objective of this work is to expand knowledge about the chemical and pharmacological properties of geopropolis, represented by its bioactive hexane fraction, conducting a study to find the compound responsible for activity on S. aureus, and a screening of pharmacological activities of the hexane fraction. Will be assessed the following properties: 1) Citototoxity, 2) bone remodeling, 3) activity of matrix metalloproteinase, 4) Antioxidant and 5) antitumor. The results will be initially submitted to an exploratory analysis to determine the best statistical test, and then applied the test more convenient for each analysis presented in this work. In the end, is expected to expand knowledge about the pharmacological potential of geopropolis, thus, a guide for the isolation and identification (s) principle (s) active (s) responsible (s) by the activity that presents the best result.

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA CUNHA, MARCOS GUILHERME; RAMOS-JUNIOR, ERIVAN SCHNAIDER; FRANCHIN, MARCELO; TAIRA, THAISE MAYUMI; BEUTLER, JOHN A.; NOBRE FRANCO, GILSON CESAR; IKEGAKI, MASAHARU; DE ALENCAR, SEVERINO MATIAS; FUKADA, SANDRA YASUYO; ROSALEN, PEDRO LUIZ. Effects of Cinnamoyloxy-mammeisin from Geopropolis on Osteoclast Differentiation and Porphyromonas gingivalis-Induced Periodontitis. Journal of Natural Products, v. 80, n. 6, p. 1893-1899, . (12/22002-2, 11/23635-6, 14/11958-3)
DA CUNHA, MARCOS GUILHERME; ROSALEN, PEDRO LUIZ; FRANCHIN, MARCELO; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; RANSOM, TANYA; BEUTLER, JOHN ALBERT. Antiproliferative Constituents of Geopropolis from the Bee Melipona scutellaris. Planta Medica, v. 82, n. 3, p. 190-194, . (12/22002-2, 11/23635-6)
NANI, BRUNO DIAS; FRANCHIN, MARCELO; LAZARINI, JOSY GOLDONI; FREIRES, IRLAN ALMEIDA; DA CUNHA, MARCOS GUILHERME; BUENO-SILVA, BRUNO; DE ALENCAR, SEVERINO MATIAS; MURATA, RAMIRO MENDONCA; ROSALEN, PEDRO LUIZ. Isoflavonoids from Brazilian red propolis down-regulate the expression of cancer-related target proteins: A pharmacogenomic analysis. Phytotherapy Research, v. 32, n. 4, p. 750-754, . (12/22378-2, 11/23635-6)
DA CUNHA, MARCOS GUILHERME; ORLANDI SARDI, JANAINA DE CASSIA; FREIRES, IRLAN ALMEIDA; FRANCHIN, MARCELO; ROSALEN, PEDRO LUIZ. Antimicrobial, anti-adherence and antibiofilm activity against Staphylococcus aureus of a 4-phenyl coumarin derivative isolated from Brazilian geopropolis. Microbial Pathogenesis, v. 139, . (11/23635-6, 12/22002-2)
DA CUNHA, MARCOS GUILHERME; NOBRE FRANCOB, GILSON CESAR; FRANCHIN, MARCELO; BEUTLER, JOHN A.; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; ROSALEN, PEDRO LUIZ. Prediction of pharmacokinetic and toxicological parameters of a 4-phenylcoumarin isolated from geopropolis: In silico and in vitro approaches. Toxicology Letters, v. 263, p. 6-10, . (12/22002-2, 11/23635-6)
PEREIRA, JOZINETE VIEIRA; FREIRES, IRLAN ALMEIDA; CASTILHO, ALINE ROG ERIA; DA CUNHA, MARCOS GUILHERME; ALVES, HARLEY DA SILVA; ROSALEN, PEDRO LUIZ. Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans. Pharmaceutical Biology, v. 54, n. 10, p. 2312-2319, . (13/25080-7, 11/23635-6, 14/01723-9)
DA CUNHA, MARCOS GUILHERME; FRANCHIN, MARCELO; DE PAULA-EDUARDO, LAILA FACIN; FREIRES, IRLAN ALMEIDA; BEUTLER, JOHN A.; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; MACHADO TABCHOURY, CINTHIA PEREIRA; CUNHA, THIAGO MATTAR; ROSALEN, PEDRO LUIZ. Anti-inflammatory and anti-biofilm properties of ent-nemorosone from Brazilian geopropolis. Journal of Functional Foods, v. 26, p. 27-35, . (12/22002-2, 12/22378-2, 11/23635-6, 11/16501-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CUNHA, Marcos Guilherme da. Isolation and identification of compounds from Melipona scutellaris geopropolis with antiproliferative and antiosteoclastogenic activities. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Campinas, SP.

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Filed patent(s) as a result of this research project

COMPOSIÇÃO FARMACÊUTICA E SEUS USOS BR1020160262569 - Universidade Estadual de Campinas (UNICAMP) ; Universidade Federal de Alfenas (UNIFAL) . Pedro Luiz Rosalen ; Marcos Guilherme Da Cunha ; Marcelo Franchin ; Masaharu Ikegaki - November 2016, 07

Composição farmacêutica e seus usos PCT/BR2017/000051 - National Institutes of Health ; Universidade Estadual de Campinas (UNICAMP) ; Universidade Federal de Alfenas (UFA) Universidade de São Paulo (USP) . Pedro Luiz Rosalen ; Masaharu Ikegaki ; John Beutler ; Thiago Mattar Cunha ; Severino Matias de Alencar ; Marcos Guilherme da Cunha ; Marcelo Franchin - May 2017, 16