Low-power laser irradiation decreases lipid droplet accumulation in the parotid glands of diabetic rats

Autor(es)

de Castro, Juliana R. ^[1] ; de Souza, Eugen M. N. ^[1] ; Park, Yun-Jong ^[2] ; de Campos, Luana ^{[1, 3]} ; Cha, Seunghee ^[2] ; Arana-Chavez, Victor E. ^[1] ; Nicolau, Jose ^[1] ; Simoes, Alyne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Lab Oral Biol, Sao Paulo - Brazil

^[2] Univ Florida, Coll Dent, Oral Maxillofacial Diagnost Sci, Gainesville, FL - USA

^[3] Brazilian Inst Canc Control, Dept Oral Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 11, n. 4 APR 2018.
• Citações Web of Science: 0

Resumo

Lipid droplet accumulation has been related to salivary gland hypofunction in diabetes. In this study, the effect of laser irradiation on the parotid glands (PGs) of diabetic rats was analyzed with regard to its effect on lipid droplet accumulation, intracellular calcium concentration and calmodulin expression. The animals were distributed into 6 groups: D0, D5, D20 and C0, C5, C20, for diabetic (D) and control animals (C), respectively. Twenty-nine days following diabetes induction, PGs of groups D5 and C5; D20 and C20 were irradiated with 5 and 20J/cm(2) of a red diode laser at 100mW, respectively. After 24hours, PGs were removed for histological, biochemical, and western blotting analysis. The diabetic animals showed lipid droplet accumulation, which was decreased after irradiation. Ultrastructurally, the droplets were nonmembrane bound and appeared irregularly located in the cytoplasm. Moreover, diabetic animals showed an increased intracellular calcium concentration. In contrast, after laser irradiation a progressive decrease in the concentration of this ion was observed, which would be in agreement with the results found in the increased expression of calmodulin in D20. These data are promising for using laser to decrease lipid droplet accumulation in PGs, however, more studies are necessary to better understand its mechanisms. Micrographs showing decreased lipid accumulation after laser irradiation in light micrographs (LM), and morphology of lipid droplet in transmission electron microscopic (TEM). LM: (A) PGs from nondiabetic rats that did not receive Laser irradiation (LI), (B) PGs from nondiabetic rats that received a dose of 20J/cm(2), (C) lipid accumulation (arrows) in the secretory cells from diabetic rats that did not receive irradiation, (D) reduction of lipid accumulation in the secretory cells from diabetic rats that received a dose of 20J/cm(2) and TEM: (E) scale bar=5 m, (F) scale bar=1 m, and (G) scale bar=0.5 mu m. (AU)

• Processo FAPESP: 12/20845-2 - Efeito do laser de baixa potência na concentração da calmodulina nas glândulas parótidas de ratas diabéticas
• Beneficiário: Juliana Rodrigues de Castro
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
• Processo FAPESP: 11/14013-1 - Estudo bioquímico e histológico comparativo do efeito das fototerapias LED, laser de baixa ou alta potência na mucosite oral e do laser na hipofunção das glândulas salivares em hamsters tratados com 5-fluorouracil
• Beneficiário: Alyne Simões Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/02739-0 - Efeito do laser de baixa potência sobre o acúmulo lipídico e concentração da calmodulina nas glândulas parótidas de ratas diabéticas
• Beneficiário: Juliana Rodrigues de Castro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica