Circulating progesterone concentrations in nonlactating Holstein cows during reuse of intravaginal progesterone implants sanitized by autoclave or chemical disinfection

Autor(es)

Melo, L. F. ^[1] ; Monteiro, Jr., P. L. J. ^[1] ; Oliveira, L. H. ^[1] ; Guardieiro, M. M. ^[2] ; Drum, J. N. ^[1] ; Wiltbank, M. C. ^[3] ; Sartori, R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anim Sci, BR-13418900 Piracicaba, SP - Brazil

^[2] Minist Agr Livestock & Food Supply, Natl Agr Lab, BR-13100105 Campinas, SP - Brazil

^[3] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 101, n. 4, p. 3537-3544, APR 2018.
• Citações Web of Science: 2

Resumo

The aim of this study was to compare plasma progesterone (P4) concentrations in nonlactating, multiparous Holstein cows (n = 24) treated with 2 types of intravaginal implants containing either 1.0 or 1.9 g of P4 either at the first use or during reuse of the implants after sanitizing the implant by autoclave or chemical disinfection. In a completely randomized design with a 2 x 3 factorial arrangement and 2 replicates, every cow underwent 2 of 6 treatments. Two sources of P4 {[}controlled internal drug release (1.9 g of P4) from Zoetis (Sao Paulo, Brazil), and Sincrogest (1.0 g of P4) from Ourofino (Cravinhos, Brazil)] and 3 types of processing, new (N), reused after autoclave (RA), and reused after chemical disinfection (RC), were used. After inducing luteolysis to avoid endogenous circulating P4, the cows were randomized in 1 of 6 treatments (1.9 g of N, 1.9 g of RA, 1.9 g of RC, 1.0 g of N, 1.0 g of RA, and 1.0 g RC). Cows were treated with the implants for 8 d and during this period blood samples were collected at 0, 2, 12, 24, 48, 72, 96, 120, 144, 168, and 192 h. Statistical analyses were performed using Proc-Mixed and the mean +/- standard error of the mean P4 concentrations were calculated using the Proc-Means procedures of SAS 9.4 (SAS Institute Inc., Cary, NC). No interaction between treatments was observed. Comparing types of implant, average P4 concentrations during treatments were greater for 1.9 g than 1.0 g (1.46 vs. 1.14 +/- 0.04 ng/mL). When types of processing were compared, average P4 concentrations did not differ between autoclaved and new inserts (1.46 vs. 1.37 +/- 0.05 ng/mL; respectively), but both were greater than chemically disinfected implants (1.09 +/- 0.04 ng/mL). Within 1.9-g P4 inserts, P4 concentrations from autoclaved implants were greater than new, which were greater than chemically disinfected (1.67 +/- 0.06 vs. 1.49 +/- 0.07 vs. 1.21 +/- 0.05 ng/mL; respectively). For 1.0-g P4 implants, P4 concentrations from autoclaved did not differ from new, but both were greater than chemically disinfected (1.20 +/- 0.08 vs. 1.24 +/- 0.06 vs. 0.97 +/- 0.05 ng/mL; respectively). In conclusion, the mean plasma P4 concentration in nonlactating Holstein cows was greater for 1.9 than 1.0 g of P4 and regardless of the type of implant, the autoclaving process provided greater circulating P4 in relation to chemical disinfection, and similar or greater P4 concentrations compared with a new implant. (AU)

• Processo FAPESP: 11/11395-0 - Aprimoramento de protocolos de manejo reprodutivo à base de hormônios exógenos para elevar a fertilidade de vacas leiteiras de alta produção
• Beneficiário: Roberto Sartori Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular