Involvement of neuronal nitric oxide synthase in cross-sensitization between chronic unpredictable stress and ethanol in adolescent and adult mice

Autor(es)

Santos-Rocha, Jaqueline Borges ^[1] ; Rae, Mariana ^[1] ; Aristimunho Teixeira, Ana Maria ^[1] ; Teixeira, Simone Aparecida ^[1] ; Munhoz, Carolina Demarchi ^[1] ; Muscara, Marcelo Nicolas ^[1] ; Marcourakis, Tania ^[2] ; Szumlinski, Karen K. ^[3] ; Camarini, Rosana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Calif Santa Barbara, Neurosci Res Inst, Dept Psychol & Brain Sci, Santa Barbara, CA 93106 - USA

• Tipo de documento: Artigo Científico
• Fonte: ALCOHOL; v. 68, p. 71-79, MAY 2018.
• Citações Web of Science: 3

Resumo

The peculiar neurochemical profile of the adolescent brain renders it differently susceptible to several stimuli, including stress and/or drug exposure. Among several stress mediators, nitric oxide (NO) has a role in stress responses. We have demonstrated that adolescent mice are less sensitive to ethanol induced sensitization than adult mice. The present study investigated whether chronic unpredictable stress (CUS) induces behavioral sensitization to ethanol in adolescent and adult Swiss mice, and investigated the influence of Ca2+-dependent nitric oxide synthase (NOS) activity in the phenomenon. Adolescent and adult mice were exposed to repeated 1.8 g/kg ethanol or CUS and challenged with saline or ethanol. A neuronal nitric oxide synthase (nNOS) inhibitor, 7-nitroindazole (7NI), was administered along with ethanol and CUS to test its effects on behavioral sensitization. Both adolescent and adult mice displayed cross-sensitization between CUS and ethanol in adult mice, with adolescents showing a lower degree of sensitization than adults. nNOS inhibition by 7NI reduced both ethanol sensitization and cross sensitization. All age differences in the Ca2+-dependent NOS activity in the hippocampus and prefrontal cortex were in the direction of greater activity in adults than in adolescents. Adolescents showed lower sensitivity to cross-sensitization between CUS and ethanol, and the nitric oxide (NO) system seems to have a pivotal role in ethanol-induced behavioral sensitization and cross-sensitization in both adolescent and adult mice. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/02397-0 - Estudo do enriquecimento ambiental como indutor de fenótipo protetor de comportamentos que acessam características aditivas em camundongos
• Beneficiário: Rosana Camarini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/50207-8 - Effects and neurobiological mechanisms of environmental manipulations on ethanol and nicotine induced behavioural effects: involvement of the stress circuitry
• Beneficiário: Rosana Camarini
• Modalidade de apoio: Auxílio à Pesquisa - Regular