Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region

Autor(es)

Souza Vieira, Jose Cavalcante ^[1] ; Braga, Camila Pereira ^[1] ; de Oliveira, Grasieli ^[1] ; Federici Padilha, Cilene do Carmo ^[1] ; de Moraes, Paula Martin ^[1] ; Zara, Luiz Fabricio ^[2] ; Leite, Aline de Lima ^[3] ; Rabelo Buzalaf, Marilia Afonso ^[3] ; Padilha, Pedro de Magalhaes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, BR-18618970 Sao Paulo - Brazil

^[2] Pontifical Catholic Univ Goias PUC, Goiania, Go - Brazil

^[3] Univ Sao Paulo, Bauru Dent Sch, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 183, n. 1, p. 164-171, MAY 2018.
• Citações Web of Science: 6

Resumo

This study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 +/- 6 and 277 +/- 4 mu g kg(-1)), followed by muscle tissues and pellets (132 +/- 4 and 86 +/- 1 mu g kg(-1), respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1-4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids. (AU)

• Processo FAPESP: 12/24035-5 - Estudo metaloproteômico do mercúrio em amostras de tecido hepático de peixes coletados na área de influência do AHE Jirau - Bacia do Rio Madeira
• Beneficiário: José Cavalcante Souza Vieira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/02668-1 - Utilização de estratégias metaloproteômicas para avaliação de metalotioneínas em amostras de tecido hepático de peixes coletados no reservatório do Ahe Jirau - Bacia do Rio Madeira, RO, Brasil
• Beneficiário: José Cavalcante Souza Vieira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Modalidade de apoio: Auxílio à Pesquisa - Regular