Biotransformation and biodegradation of methyl parathion by Brazilian bacterial strains isolated from mangrove peat

Autor(es)

Alvarenga, Natalia ^[1] ; Birolli, Willian G. ^[1] ; Meira, Eloa B. ^[1] ; Lucas, Simone C. O. ^[2] ; de Matos, Iara L. ^[1] ; Nitschke, Marcia ^[1] ; Romao, Luciane P. C. ^[2] ; Porto, Andre L. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Ave Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Fed Sergipe, Ave Marechal Rondon S-N, BR-49100000 Sao Cristovao, Sergipe - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 13, p. 319-326, JAN 2018.
• Citações Web of Science: 2

Resumo

Four bacterial strains (Bacillus sp. CBMAI 1833, Bacillus cereus P5CNB, Kosakonia sp. CBMAI 1836 and Kosakonia sp. CBMAI 1835) isolated from a Brazilian mangrove peat were evaluated for biodegradation of methyl parathion. The strains Bacillus sp. CBMAI 1833 and B. cereus P5CNB showed a better methyl parathion degradation at 36 h than the Kosakonia strains. By HPLC-UV analysis, it was observed in the presence of both strains that all methyl parathion was biotransformed and biodegraded in 24 h of incubation. HPLC-ToF and GC-MS analysis were employed for identification of metabolites from the methyl parathion reactions. The first reaction of the biodegradation pathway was the direct hydrolysis of the pesticide to yield p-nitrophenol by a phase I reaction. The biotransformation of methyl parathion occurs via the nitro group reduction with the formation of an amine group in the phenolic moiety, followed by the amine acetylation to yield an acetamide derivative by phase II reactions. Further biodegradation proceeded with the hydrolysis of the acetamide product forming N-(4-hydroxyphenyl) acetamide. Bacillus sp. CBMAI 1833 and B. cereus P5CNB were also able to promote the reduction of p-nitrophenol levels in 12 days, showing potential for future bioremediation studies. (AU)

• Processo FAPESP: 04/09498-2 - Caracterização estrutural, identificação e quantificação de substâncias orgânicas e moléculas bioativas por cromatografia em fase líquida acoplada a espectrometria de massas tandem (LC-MS/MS) com equipamento híbrido quadrupolo-tempo de voo
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 12/19934-0 - Biodegradação de pesticidas por micro-organismos (fungos e bactérias)
• Beneficiário: Andre Luiz Meleiro Porto
• Modalidade de apoio: Auxílio à Pesquisa - Regular