Photochemoprotective effect of a fraction of a partially purified extract of Byrsonima crassifolia leaves against UVB-induced oxidative stress in fibroblasts and hairless mice

Autor(es)

de Souza, Rebeca Oliveira ^[1] ; Dias Alves, Georgia de Assis ^[1] ; Scarano Aguillera, Ana Luiza ^[1] ; Rogez, Herve ^{[2, 3]} ; Vieira Fonseca, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Ctr Agrofood Valorizat Amazonian Bioact Cpds CVAC, R Augusto Correa 1, BR-6607511 Belem, Para - Brazil

^[3] Fed Univ Para, R Augusto Correa 1, BR-6607511 Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 178, p. 53-60, JAN 2018.
• Citações Web of Science: 5

Resumo

Ultraviolet B (UVB) irradiation increases the risk of various skin disorders, leading to inflammation and oxidative stress and thereby increasing the risk of skin photoaging and carcinogenesis. The use of photo-chemoprotectors such as natural products with antioxidant and anti-inflammatory properties represents a strategy for preventing UVB-induced skin damage. We investigated the photochemoprotective effect of a fraction of a partially purified extract of Byrsonima crassifolia leaves (BCF) on fibroblasts and hairless mice exposed to UVB radiation. The mixture of phenolic compounds in BCF prevented the decrease in reduced glutathione (GSH) levels in fibroblast cultures induced by UVB radiation more than some of their individual standards ((+)-catechin (CAT), epigallocatechin gallate and quercetin 3-O-beta-D-glucopyranoside). Prepared gel formulations increased skin antioxidant activity, and BCF components and the CAT standard were retained in the HRS/J hairless mice epidermis 2 h after application. Topical treatment with the BCF or CAT formulations (1%) significantly reduced the decrease in GSH levels and decreased myeloperoxidase activity and the secretion of pro-inflammatory cytokines IL-1 beta and IL-6 induced by UVB radiation (P < 0.05), indicating that both BCF and CAT had anti-inflammatory effects. BCF inhibited UVB-induced metalloproteinase (MMP)-9 secretion/activity, whereas CAT had no effect on MMP-9 activity in the skin of treated animals. These results therefore suggest that BCF can be used as a photochemoprotective agent and antioxidant in the prevention/treatment of inflammation and oxidative stress of the skin induced by UVB radiation. (AU)

• Processo FAPESP: 08/00216-5 - Avaliação in vivo e in vitro da atividade fotoquimiopreventiva de extratos vegetais
• Beneficiário: Maria José Vieira Fonseca
• Modalidade de apoio: Auxílio à Pesquisa - Regular