Mass spectrometry characterization of endophytic bacterium Curtobacterium sp strain ER1/6 isolated from Citrus sinensis

Autor(es)

da Silva Araujo, Francisca Diana ^[1] ; Santos, Daiene Souza ^[2] ; Pagotto, Carolina Clepf ^[1] ; de Araujo, Welington Luiz ^[2] ; Eberlin, Marcos Nogueira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, POB 6154, BR-13084970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Mass Spectrometry; v. 53, n. 1, p. 91-97, JAN 2018.
• Citações Web of Science: 0

Resumo

The bacteria of the genus Curtobacterium are usually seen as plant pathogen, but some species have been identified as endophytes of different crops and could as such present a potential for disease control and plant growth promotion. We have therefore applied the desorption electrospray ionization mass spectrometry imaging (DESI-MSI) in the direct analysis of living Curtobacterium sp. strain ER1/6 colonies to map the surface metabolites, and electrospray ionization tandem mass spectrometry (ESI-MS/MS) for characterization of these compounds. Several colony-associated metabolites were detected. The ESI-MS/MS showed characteristic fragmentations for phospholipids including the classes of glycerophosphocholine, glycerophosphoglycerol, and glycerophosphoinositol as well as several fatty acids. Although a secure identification was not obtained, many other metabolites were also detected for this bacteria species. Principal component analysis showed that fatty acids were discriminatory for Curtobacterium sp. ER1/6 during inoculation on periwinkle wilt (PW) medium, whereas phospholipids characterize the bacterium when grown on the tryptic soy agar (TSA) medium. (AU)

• Processo FAPESP: 15/07659-3 - Aplicação de espectrometria de massas na análise metabolômica do oomiceto de citros Phytophthora parasitica: identificação de biomarcadores
• Beneficiário: Carolina Clepf Pagotto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/24860-9 - Investigação de trocas metabólicas entre micro-organismos endofíticos e patogênicos de Theobroma cacao e Citrus sinensis utilizando imageamento por espectrometria de massas
• Beneficiário: Francisca Diana da Silva Araújo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado