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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mass spectrometry characterization of endophytic bacterium Curtobacterium sp strain ER1/6 isolated from Citrus sinensis

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Author(s):
da Silva Araujo, Francisca Diana [1] ; Santos, Daiene Souza [2] ; Pagotto, Carolina Clepf [1] ; de Araujo, Welington Luiz [2] ; Eberlin, Marcos Nogueira [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, POB 6154, BR-13084970 Campinas, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Mass Spectrometry; v. 53, n. 1, p. 91-97, JAN 2018.
Web of Science Citations: 0
Abstract

The bacteria of the genus Curtobacterium are usually seen as plant pathogen, but some species have been identified as endophytes of different crops and could as such present a potential for disease control and plant growth promotion. We have therefore applied the desorption electrospray ionization mass spectrometry imaging (DESI-MSI) in the direct analysis of living Curtobacterium sp. strain ER1/6 colonies to map the surface metabolites, and electrospray ionization tandem mass spectrometry (ESI-MS/MS) for characterization of these compounds. Several colony-associated metabolites were detected. The ESI-MS/MS showed characteristic fragmentations for phospholipids including the classes of glycerophosphocholine, glycerophosphoglycerol, and glycerophosphoinositol as well as several fatty acids. Although a secure identification was not obtained, many other metabolites were also detected for this bacteria species. Principal component analysis showed that fatty acids were discriminatory for Curtobacterium sp. ER1/6 during inoculation on periwinkle wilt (PW) medium, whereas phospholipids characterize the bacterium when grown on the tryptic soy agar (TSA) medium. (AU)

FAPESP's process: 15/07659-3 - Mass spectrometry applications in metabolomic analysis of Phytophthora parasitic citrus oomycete: biomarkers identification
Grantee:Carolina Clepf Pagotto
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/24860-9 - Investigation of metabolic exchanges between pathogenic and endophytic microorganisms from Theobroma cacao and citrus sinensis by imaging mass spectrometry
Grantee:Francisca Diana da Silva Araújo
Support Opportunities: Scholarships in Brazil - Post-Doctoral