Investigation of metabolic exchanges between pathogenic and endophytic microorganisms from Theobroma cacao and citrus sinensis by imaging mass spectrometry

• Grant number: 13/24860-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): July 01, 2014
• Effective date (End): June 30, 2016
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcos Nogueira Eberlin
• Grantee: Francisca Diana da Silva Araújo
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Metabolic exchanges between an organism and the environment, including interactions with neighboring organisms, are very important to the development processes of the body. Such interactions have been studied for long time, however, by methods that not provide spatial and temporal analyses of secondary metabolites in biological samples. In this context, the imaging mass spectrometry (IMS) emerges as a powerful tool that allows the observation of the effects of multiple microbial signals in an interspecies interaction, enabling an advanced understanding of the chemical signaling process. Thus, in this project, IMS will be applied in the investigation of metabolic exchanges that occur during interactions between fungal pathogens of the cocoa (Theobroma cacao L.) and fungal endophyte Clonostachys rosea, potential biocontrol agent, and in interactions between endophytic bacteria from citrus plant and the phytopathogenic bacteria Xylella fastidiosa, causal agent of citrus-variegated chlorosis (CVC) that occur in sweet orange (Citrus sinensis L.) and other commercial citrus. Given that these pathogens are responsible for significant economic losses in Brazilian cocoa plantations and citriculture, the results obtained in this proposal will be of paramount importance for understanding of the defense mechanisms of these microorganisms, and may provide grants to combat the diseases caused by phytopathogens. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA ARAUJO, FRANCISCA DIANA; SANTOS, DAIENE SOUZA; PAGOTTO, CAROLINA CLEPF; DE ARAUJO, WELINGTON LUIZ; EBERLIN, MARCOS NOGUEIRA. Mass spectrometry characterization of endophytic bacterium Curtobacterium sp strain ER1/6 isolated from Citrus sinensis. Journal of Mass Spectrometry, v. 53, n. 1, p. 91-97, JAN 2018. (15/07659-3, 13/24860-9)

ARAUJO, F. D. S.; VIEIRA, R. L.; MOLANO, E. P. L.; MAXIMO, H. J.; DALIO, R. J. D.; VENDRAMINI, P. H.; ARAUJO, W. L.; EBERLIN, M. N.. Desorption electrospray ionization mass spectrometry imaging reveals chemical defense of Burkholderia seminalis against cacao pathogens. RSC ADVANCES, v. 7, n. 48, p. 29953-29958, 2017. (13/24860-9, 15/18454-3)

DA SILVA ARAUJO, FRANCISCA DIANA; ARAUJO, WELINGTON LUIZ; EBERLIN, MARCOS NOGUEIRA. Potential of Burkholderia seminalis TC3.4.2R3 as Biocontrol Agent Against Fusarium oxysporum Evaluated by Mass Spectrometry Imaging. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v. 28, n. 5, p. 901-907, MAY 2017. (13/24860-9, 15/11563-1)