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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight

Texto completo
Autor(es):
Vaz, Juliana M. [1] ; Taketa, Thiago B. [1] ; Hernandez-Montelongo, Jacobo [2, 3] ; Chevallier, Pascale ; Cotta, Monica A. [2] ; Mantovani, Diego [4, 5] ; Beppu, Marisa M. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Fac Engn Quim, Dept Engn Mat & Bioproc DEMBio, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, Campinas, SP - Brazil
[3] Univ Catolica Temuco, Fac Ingn, Dept Ciencias Matemat & Fis, Temuco, La Araucania - Chile
[4] Laval Univ, Dept Min Met & Mat Engn, Lab Biomat & Bioengn, Quebec City, PQ - Canada
[5] Laval Univ, CHU Quebec Res Ctr, Quebec City, PQ - Canada
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Applied Surface Science; v. 445, p. 478-487, JUL 1 2018.
Citações Web of Science: 3
Resumo

Chitosan is a biopolymer with antibacterial properties, which are dependent on its molecular weight (Mw) and its degree of deacetylation (DDA). When grafted on surfaces as a coating, chitosan antibacterial efficiency is also dependent on the polymer chain conformation on the surface, as the amine groups, responsible of the antibacterial effect, should be available for contact with bacteria. To investigate this behavior, chitosans with different Mw were grafted onto plasma aminated surfaces through three different spacers: glutaric anhydride (GA), poly(ethylene-glycol) bis(carboxymethyl) ether (PEGb), and poly (ethylene-alt-maleic anhydride) (PA). The grafting efficiency was evaluated by X-ray Photoelectron Spectroscopy (XPS), contact angle and Rose Bengal test, while morphological features were assessed by profilometry analyses. Results evidenced a clear influence of the anchor arm length and of the Mw of chitosan both on the grafting efficiency and on the antibacterial behavior. PA CHIMW surface exhibited a better antibacterial response compared to GA and PEGb, which could be correlated to a denser coating coverage as seen by XPS and profilometry results. Further, PA CHIMW coating displayed a higher amine density, thus promoting the interaction with the bacteria cell wall. Based on these results, chitosan-based coatings can then be extended to a wide range of antibacterial applications. (C) 2018 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
Beneficiário:Alessandra Alves de Souza
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/05135-1 - Recobrimentos antimicrobianos e nanoestruturados contendo quitosana produzidos pela técnica de automontagem (layer-by-layer) para substratos têxteis
Beneficiário:Thiago Bezerra Taketa
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa
Beneficiário:Mônica Alonso Cotta
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/14888-3 - Comportamento da adesão das bactérias Pseudomonas aeruginosa e staphylococcus aureus em filmes nanoestruturados contendo quitosana: estudos na nano e microescalas
Beneficiário:Jesús Jacobo Hernández Montelongo
Linha de fomento: Bolsas no Brasil - Pós-Doutorado