Evaluation of peptides release using a natural rubber latex biomembrane as a carrier

Autor(es)

Miranda, M. C. R. ^{[1, 2]} ; Borges, F. A. ^{[1, 2]} ; Barros, N. R. ^{[1, 2]} ; Santos Filho, N. A. ^[1] ; Mendonca, R. J. ^[3] ; Herculano, R. D. ^{[1, 2]} ; Cilli, E. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Quim, UNESP, Rua Prof Francisco Degni 55, BR-14800060 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Bioproc & Biotechnol Dept, FCF, UNESP, Sao Paulo - Brazil

^[3] Univ Fed Triangulo Mineiro, Cincias Biol Dept, Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 50, n. 5, p. 503-511, MAY 2018.
• Citações Web of Science: 3

Resumo

The biomembrane natural (NRL-Natural Rubber Latex), manipulated from the latex obtained from the rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Reflecting the biocompatibility and low bounce rate of this material, NRL has been used as a physical barrier to infectious agents and for the controlled release of drugs and extracts. The aim of the present study was to evaluate the incorporation and release of peptides using a latex biomembrane carrier. After incorporation, the release of material from the membrane was observed using spectrophotometry. Analyses using HPLC and mass spectroscopy did not confirm the release of the antimicrobial peptide {[}W-6]Hylin a1 after 24 h. In addition, analysis of the release solution showed new compounds, indicating the degradation of the peptide by enzymes contained in the latex. Additionally, the release of a peptide with a shorter sequence (Ac-WAAAA) was evaluated, and degradation was not observed. These results showed that the use of NRL as solid matrices as delivery systems of peptide are sequence dependent and could to be evaluated for each sequence. (AU)

• Processo FAPESP: 12/15346-7 - Peptídeos antimicrobianos: efeito da dimerização e produção de biomembranas de látex/peptídeos
• Beneficiário: Eduardo Maffud Cilli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17411-8 - Avaliação da liberação controlada de fármacos utilizando membranas de látex natural como carreador
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/02343-8 - Liberação controlada de peptídeos em biomembranas de látex e desenvolvimento de biossensores capacitivos constituídos de monocamadas peptídicas nanometricamente estruturadas
• Beneficiário: Eduardo Maffud Cilli
• Modalidade de apoio: Auxílio à Pesquisa - Regular