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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection

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Autor(es):
Rodrigues, Tasson C. [1] ; Oliveira, Maria Leonor S. [1] ; Soares-Schanoski, Alessandra [1] ; Chavez-Rico, Stefanni L. [1] ; Figueiredo, Douglas B. [2] ; Goncalves, Viviane M. [2] ; Ferreira, Daniela M. [3] ; Kunda, Nitesh K. [4, 5] ; Saleem, Imran Y. [5] ; Miyaji, Eliane N. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Bacteriol, Sao Paulo, SP - Brazil
[2] Inst Butantan, Lab Especial Desenvolvimento Vacinas, Sao Paulo, SP - Brazil
[3] Univ Liverpool Liverpool Sch Trop Med, Resp Infect Grp, Liverpool, Merseyside - England
[4] Univ New Mexico, Coll Pharm, Dept Pharmaceut Sci, Albuquerque, NM 87131 - USA
[5] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Formulat & Drug Delivery Res, Liverpool, Merseyside - England
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 13, n. 1 JAN 23 2018.
Citações Web of Science: 11
Resumo

Burden of pneumonia caused by Streptococcus pneumoniae remains high despite the availability of conjugate vaccines. Mucosal immunization targeting the lungs is an attractive alternative for the induction of local immune responses to improve protection against pneumonia. Our group had previously described the development of poly(glycerol adipate-co-omega-pentadecalactone) (PGA-co-PDL) polymeric nanoparticles (NPs) adsorbed with Pneumococcal surface protein A from clade 4 (PspA4Pro) within L-leucine microcarriers (nanocomposite microparticles-NCMPs) for mucosal delivery targeting the lungs (NP/NCMP PspA4Pro). NP/NCMP PspA4Pro was now used for immunization of mice. Inoculation of this formulation induced anti-PspA4Pro IgG antibodies in serum and lungs. Analysis of binding of serum IgG to intact bacteria showed efficient binding to bacteria expressing PspA from clades 3, 4 and 5 (family 2), but no binding to bacteria expressing PspA from clades 1 and 2 (family 1) was observed. Both mucosal immunization with NP/NCMP PspA4Pro and subcutaneous injection of the protein elicited partial protection against intranasal lethal pneumococcal challenge with a serotype 3 strain expressing PspA from clade 5 (PspA5). Although similar survival levels were observed for mucosal immunization with NP/NCMP PspA4Pro and subcutaneous immunization with purified protein, NP/NCMP PspA4Pro induced earlier control of the infection. Conversely, neither immunization with NP/NCMP PspA4Pro nor subcutaneous immunization with purified protein reduced bacterial burden in the lungs after challenge with a serotype 19F strain expressing PspA from clade 1 (PspA1). Mucosal immunization with NP/NCMP PspA4Pro targeting the lungs is thus able to induce local and systemic antibodies, conferring protection only against a strain expressing PspA from the homologous family 2. (AU)

Processo FAPESP: 15/06255-6 - Desenvolvimento e produção de novas vacinas pneumocócicas baseadas em proteínas recombinantes
Beneficiário:Viviane Maimoni Gonçalves
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/25436-9 - Nanopartículas contendo o antígeno PspA (Pneumococcal surface protein A) como vacina contra pneumonia pneumocócica
Beneficiário:Tasson da Costa Rodrigues
Linha de fomento: Bolsas no Brasil - Iniciação Científica