Search for independent (beta/alpha)(4) subdomains in a (beta/alpha)(8) barrel beta-glucosidase

Autor(es)

Almeida, Vitor M. ^[1] ; Frutuoso, Maira A. ^[1] ; Marana, Sandro R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 1 JAN 16 2018.
• Citações Web of Science: 1

Resumo

Proteins that fold as (beta/alpha)(8) barrels are thought to have evolved from half-barrels that underwent duplication and fusion events. The evidence is particularly clear for small barrels, which have almost identical halves. Additionally, computational calculations of the thermodynamic stability of these structures in the presence of denaturants have revealed that (beta/alpha)(8) barrels contain two subunits or domains corresponding to half -barrels. Hence, within (beta/alpha)(8) barrels, half -barrels are self-contained units. Here, we tested this hypothesis using 6-glucosidase from the bacterium Thermotoga maritima (bglTm), which has a (beta/alpha)(8) barrel structure. Mutations were introduced to disrupt the noncovalent contacts between its halves and reveal the presence of two domains within bglTm, thus resulting in the creation of mutants T1 (containing W12A and I217A mutations) and T2 (containing W12A, H195A, I217A and F404A mutations). Mutants T1 and T2 were properly folded, as indicated by their fluorescence spectra and enzyme kinetic parameters. T1 and wild-type bglTm were equally stable, as shown by the results of thermal inactivation, differential scanning fluorimetry and guanidine hydrochloride denaturation experiments. However, T2 showed a first-order inactivation at 80 degrees C, a single melting temperature of 82 degrees C and only one transition concentration (c(50)) in 2.4 M guanidine hydrochloride. Additionally, T1 and T2 exhibited a cooperative denaturation process that followed a two-state model (m-values equal to 1.4 and 1.6 kcal/mol/M, respectively), similar to that of wild-type bglTm (1.2 kcal/mol/M). Hence, T1 and T2 each denatured as a single unit, although they contained different degrees of disruption between their halves. In conclusion, bglTm halves are equivalent in terms of their thermal and chemical stability; thus, their separate contributions to (beta/alpha)(8) barrel unfolding cannot be disentangled. (AU)

• Processo FAPESP: 14/19439-5 - Redes estruturais e propriedades funcionais em enzimas
• Beneficiário: Sandro Roberto Marana
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/22365-9 - Redes estruturais e dinâmica em enzimas
• Beneficiário: Sandro Roberto Marana
• Linha de fomento: Auxílio à Pesquisa - Regular