A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome

Autor(es)

Leite Armanhi, Jaderson Silveira ^{[1, 2]} ; Correa de Souza, Rafael Soares ^{[1, 2]} ; Damasceno, Natalia de Brito ^{[1, 2]} ; de Araujo, Laura M. ^{[1, 2]} ; Imperial, Juan ^{[3, 4]} ; Arruda, Paulo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP - Brazil

^[3] Univ Politecn Madrid, Inst Nacl Invest & Tecnol Agr & Alimentaria, Ctr Biotecnol & Genom Plantas, Madrid - Spain

^[4] CSIC, Madrid - Spain

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 8, JAN 4 2018.
• Citações Web of Science: 9

Resumo

The soil-plant ecosystem harbors an immense microbial diversity that challenges investigative approaches to study traits underlying plant-microbe association. Studies solely based on culture-dependent techniques have overlooked most microbial diversity. Here we describe the concomitant use of culture-dependent and -independent techniques to target plant-beneficial microbial groups from the sugarcane microbiome. The community-based culture collection (CBC) approach was used to access microbes from roots and stalks. The CBC recovered 399 unique bacteria representing 15.9% of the rhizosphere core microbiome and 61.6-65.3% of the endophytic core microbiomes of stalks. By cross-referencing the CBC (culture-dependent) with the sugarcane microbiome profile (culture-independent), we designed a synthetic community comprised of naturally occurring highly abundant bacterial groups from roots and stalks, most of which has been poorly explored so far. We then used maize as a model to probe the abundance-based synthetic inoculant. We show that when inoculated in maize plants, members of the synthetic community efficiently colonize plant organs, displace the natural microbiota and dominate at 53.9% of the rhizosphere microbial abundance. As a result, inoculated plants increased biomass by 3.4-fold as compared to uninoculated plants. The results demonstrate that abundance-based synthetic inoculants can be successfully applied to recover beneficial plant microbes from plant microbiota. (AU)

• Processo FAPESP: 16/04322-0 - Identificação de relações microrganismo/microrganismo e microrganismo/planta à promoção do crescimento vegetal a partir de uma coleção de bactérias representativa do microbioma da cana-de-açúcar
• Beneficiário: Jaderson Silveira Leite Armanhi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/23425-5 - Mapeamento da colonização de plantas de milho e de soja por uma comunidade microbiana sintética oriunda do microbioma da cana-de-açúcar
• Beneficiário: Natália de Brito Damasceno
• Modalidade de apoio: Bolsas no Brasil - Mestrado