| Full text | |
| Author(s): |
Leite Armanhi, Jaderson Silveira
[1, 2]
;
Correa de Souza, Rafael Soares
[1, 2]
;
Damasceno, Natalia de Brito
[1, 2]
;
de Araujo, Laura M.
[1, 2]
;
Imperial, Juan
[3, 4]
;
Arruda, Paulo
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP - Brazil
[3] Univ Politecn Madrid, Inst Nacl Invest & Tecnol Agr & Alimentaria, Ctr Biotecnol & Genom Plantas, Madrid - Spain
[4] CSIC, Madrid - Spain
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 8, JAN 4 2018. |
| Web of Science Citations: | 9 |
| Abstract | |
The soil-plant ecosystem harbors an immense microbial diversity that challenges investigative approaches to study traits underlying plant-microbe association. Studies solely based on culture-dependent techniques have overlooked most microbial diversity. Here we describe the concomitant use of culture-dependent and -independent techniques to target plant-beneficial microbial groups from the sugarcane microbiome. The community-based culture collection (CBC) approach was used to access microbes from roots and stalks. The CBC recovered 399 unique bacteria representing 15.9% of the rhizosphere core microbiome and 61.6-65.3% of the endophytic core microbiomes of stalks. By cross-referencing the CBC (culture-dependent) with the sugarcane microbiome profile (culture-independent), we designed a synthetic community comprised of naturally occurring highly abundant bacterial groups from roots and stalks, most of which has been poorly explored so far. We then used maize as a model to probe the abundance-based synthetic inoculant. We show that when inoculated in maize plants, members of the synthetic community efficiently colonize plant organs, displace the natural microbiota and dominate at 53.9% of the rhizosphere microbial abundance. As a result, inoculated plants increased biomass by 3.4-fold as compared to uninoculated plants. The results demonstrate that abundance-based synthetic inoculants can be successfully applied to recover beneficial plant microbes from plant microbiota. (AU) | |
| FAPESP's process: | 16/04322-0 - Cross-talk between microorganism-microorganism and plant-microorganism from a representative bacterial collection of the sugarcane microbiome |
| Grantee: | Jaderson Silveira Leite Armanhi |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 16/23425-5 - Mapping the colonization of a synthetic microbial community inoculum from sugarcane microbiome in maize and soybean plants |
| Grantee: | Natália de Brito Damasceno |
| Support Opportunities: | Scholarships in Brazil - Master |