Analysis of bacterial volatiles as plant growth promoters

• Grant number: 18/04184-2
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): May 01, 2018
• Effective date (End): July 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Juliana Velasco de Castro Oliveira
• Grantee: Bruno Henrique Silva Dias
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated scholarship(s): 19/19121-9 - Identification of bacterial volatiles that promotes plant growth through HS-SPME/GC-MS, BE.EP.MS

Abstract

Despite the advances on agricultural practices to feed the growing world, it is necessary to increase the global food production adopting sustainable practices. Several plant growth-promoting bacteria can greatly increase crop yield through a wide variety of interaction mechanisms. Recently, our group have isolated more than 7,000 bacteria from sugarcane roots or cultivated soil in different regions, and about 100 were able to produce higher quantity of the hormone auxin and/or solubilize phosphate. Furthermore, in a preliminary assay, we were able to identify species producing volatile organic compounds (VOCs), which were able to promote plant growth. The use of microorganisms VOCs to promote plant growth is promising, since it does not depend on the direct contact and plant colonization, making them ideal molecules for mediating short- and long-distance organism interactions. In model plants, such as Arabidopsis thaliana, bacterial VOCs has been recently considered as an important feature to enhance plant growth and health. However, so far, there are only very few studies showing the impact of bacterial VOCs on important agricultural crops. In this scenario, one of the more precise and used platform that allow these VOCs profiles identification is based on metabolomics. Accordingly, the main objectives of this project are to: (i) establish a metabolomics robust pipeline based on HS-GC/MS to identify and quantify VOCs, (ii) evaluate the potential of different bacteria genera from our collection to promote plant growth mediated by VOCs, (iii) test these VOCs efficiency in sugarcane cultures, and (iv) identify if specific VOCs can promote the plant growth. As a long-term goal, we expect to develop bioinoculants as a sustainable approach to replace or reduce chemical supplies and increase plant productivity as well as reduce food production costs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, OCTAVIO AUGUSTO COSTA; DE ARAUJO, NATALIA OLIVEIRA; DIAS, BRUNO HENRIQUE SILVA; FREITAS, CARLA DE SANT'ANNA; COERINI, LUCIANE FENDER; RYU, CHOONG-MIN; OLIVEIRA, JULIANA VELASCO DE CASTRO. The power of the smallest: The inhibitory activity of microbial volatile organic compounds against phytopathogens. FRONTIERS IN MICROBIOLOGY, v. 13, p. 31-pg., 2023-01-04. (18/04184-2, 17/24395-5, 22/00474-1, 19/08522-2)

SILVA DIAS, BRUNO HENRIQUE; JUNG, SUNG-HEE; DE CASTRO OLIVEIRA, JULIANA VELASCO; RYU, CHOONG-MIN. C4 Bacterial Volatiles Improve Plant Health. PATHOGENS, v. 10, n. 6, JUN 2021. (18/04184-2, 17/20521-6, 19/19121-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DIAS, Bruno Henrique Silva. Analysis of bacterial volatiles that promote plant growth. 2021. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.