| Texto completo | |
| Autor(es): |
Tozetto-Mendoza, Tania R.
[1]
;
Sumita, Laura M.
[1]
;
Palmieri, Michelle
[2]
;
Ornaghi, Mariana
[2]
;
do Canto, Alan M.
[2]
;
Braz-Silva, Paulo H.
[1, 2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF INVESTIGATIVE AND CLINICAL DENTISTRY; v. 9, n. 1 FEB 2018. |
| Citações Web of Science: | 2 |
| Resumo | |
Aim: Saliva can play an important role in human herpesvirus-8 (HHV-8) transmission in endemic regions for Kaposi's sarcoma (KS). Little is known about HHV-8 oral shedding in immunocompetent individuals from non-endemic regions for KS. Methods: We conducted a prospective study of HHV-8 salivary excretion among 59 healthy, immunocompetent individuals from SAo Paulo, Brazil, followed up weekly for 4 months, resulting in 16 saliva samples from each participant. Antibodies to HHV-8 latency-associated nuclear antigen (LANA) and lytic-phase antigens were investigated with immunofluorescence assays (IFA). HHV-8 DNA detection was performed using real-time polymerase chain reaction (PCR). Results: All 59 individuals were seronegative to LANA and lytic antibodies. HHV-8 DNA was undetectable in saliva samples in 100% of the participants, totaling 944 samples and being consistently negative during the different periods of sampling, which lasted approximately 120 days. No sequences of HHV-8 DNA were detected in the saliva samples of healthy, immunocompetent adults by using real-time PCR, with the resulting data being consistent with IFA-based serological tests. Conclusions: Unlike other herpesviruses, HHV-8 is not excreted in the saliva of healthy individuals from non-endemic regions for KS. (AU) | |
| Processo FAPESP: | 12/04223-1 - Dinâmica da excreção oral e viremia do herpesvírus 8 humano (HHV-8) em indivíduos HIV positivos e negativos |
| Beneficiário: | Claudio Sergio Pannuti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/04303-5 - Dinâmica da excreção oral e viremia do herpesvírus 8 humano (HHV-8) em indivíduos HIV positivos e negativos |
| Beneficiário: | Paulo Henrique Braz da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |