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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

No detectable human herpesvirus-8 oral shedding in seronegative-healthy, immunocompetent individuals from non-endemic regions for Kaposi's sarcoma: A pilot study

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Author(s):
Tozetto-Mendoza, Tania R. [1] ; Sumita, Laura M. [1] ; Palmieri, Michelle [2] ; Ornaghi, Mariana [2] ; do Canto, Alan M. [2] ; Braz-Silva, Paulo H. [1, 2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF INVESTIGATIVE AND CLINICAL DENTISTRY; v. 9, n. 1 FEB 2018.
Web of Science Citations: 2
Abstract

Aim: Saliva can play an important role in human herpesvirus-8 (HHV-8) transmission in endemic regions for Kaposi's sarcoma (KS). Little is known about HHV-8 oral shedding in immunocompetent individuals from non-endemic regions for KS. Methods: We conducted a prospective study of HHV-8 salivary excretion among 59 healthy, immunocompetent individuals from SAo Paulo, Brazil, followed up weekly for 4 months, resulting in 16 saliva samples from each participant. Antibodies to HHV-8 latency-associated nuclear antigen (LANA) and lytic-phase antigens were investigated with immunofluorescence assays (IFA). HHV-8 DNA detection was performed using real-time polymerase chain reaction (PCR). Results: All 59 individuals were seronegative to LANA and lytic antibodies. HHV-8 DNA was undetectable in saliva samples in 100% of the participants, totaling 944 samples and being consistently negative during the different periods of sampling, which lasted approximately 120 days. No sequences of HHV-8 DNA were detected in the saliva samples of healthy, immunocompetent adults by using real-time PCR, with the resulting data being consistent with IFA-based serological tests. Conclusions: Unlike other herpesviruses, HHV-8 is not excreted in the saliva of healthy individuals from non-endemic regions for KS. (AU)

FAPESP's process: 12/04223-1 - HHV-8 oral shedding and viremia in HIV positive and negative patients
Grantee:Claudio Sergio Pannuti
Support Opportunities: Regular Research Grants
FAPESP's process: 12/04303-5 - Humam Herpesvirus 8 (HHV-8)oral shedding and viremia in HIV positive and negative patients.
Grantee:Paulo Henrique Braz da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral