Development of microsatellite markers for Myracrodruon urundeuva (FF & MF Allemao), a highly endangered species from tropical forest based on next-generation sequencing

Autor(es)

Lemos Souza, Danilla Cristina ^[1] ; Rossini, Bruno Cesar ^{[2, 3]} ; de Souza, Francine Beatriz ^[4] ; Sebbenn, Alexandre Magno ^[5] ; Marino, Celso Luis ^{[2, 3]} ; Teixeira de Moraes, Mario Luiz ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Agron, CP 237, BR-18610307 Botucatu, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Biotechnol Inst IBTEC, BR-18607440 Botucatu, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Genet, BR-18618689 Botucatu, SP - Brazil

^[4] UNESP Univ Estadual Paulista, Fac Engn, BR-15385000 Ilha Solteira, SP - Brazil

^[5] Sao Paulo Forestry Inst, CP 1322, BR-01059970 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 45, n. 1, p. 71-75, FEB 2018.
• Citações Web of Science: 1

Resumo

Myracrodruon urundeuva is a tree species of high economic importance due the strength and durability of its wood. Threatened of extinction in Brazil, it is present only in a few forest remnants, mostly in conservation units. Currently, there is little information on the genetic diversity of natural populations in Brazil and even less information about the genome of this species. Here, new species-specific microsatellite loci were developed based on next-generation sequencing (Illumina). More than 100,000 loci were identified in the run, with di- to hexanucleotides motifs. Of these, 20 loci were selected for validation in 30 individuals, with 15 successfully polymorphic loci detected. The number of alleles ranged among loci from 3 to 16, with an average of 7.73, expected (H-e ) and observed (H-o ) heterozygosity ranged from 0.246 to 0.902 and from 0.103 to 0.867, respectively. These results point out that these new set of markers has a great potential for use in population genetic studies for genetic conservation of the species. (AU)

• Processo FAPESP: 13/24503-1 - Sistema de reprodução e distribuição da variabilidade genética de Myracrodruon urundeuva (F.F. & M.F. Allemão) em diferentes biomas
• Beneficiário: Danilla Cristina Lemos Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado