Binding of human complement C1 sterase inhibitor to Leptospira spp.

Autor(es)

Dantas Breda, Leandro Carvalho ^[1] ; Vasconcellos, Silvio Arruda ^[2] ; Vasconcelos, Dewton de Moraes ^{[3, 4]} ; Isaac, Lourdes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Av Prof. Linen Prestes 1730, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Dermatol, Ambulatory Dermatol Manifestat Immunodeficiencies, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Immunodeficiencies LIM 56, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunobiology; v. 223, n. 2, p. 183-190, FEB 2018.
• Citações Web of Science: 0

Resumo

Leptospirosis is an important zoonosis of global importance caused by bacteria Leptospira spp. Pathogenic Leptospira is resistant to Complement System killing while non-pathogenic Leptospira is rapidly killed by exposure to normal human serum (NHS). Pathogenic Leptospira interact with Complement Regulators such as Factor H, C4b binding protein and Vitronectin avoiding Complement activation and killing by Alternative and Classical Pathways. One important regulator is C1-inhibitor (C1INH) that interacts with C1s or MASPs controlling the cleavage of C4 and C2 molecules, thereby inhibiting the activation of the Classical and Lectin Pathways. In this study, we demonstrate that attenuated, saprophytic and pathogenic Leptospira interact with C1INH that maintain its regulatory capacity of interaction with C1s preventing the activation of Complement system. Although the interaction with C1INH is not crucial for pathogenic Leptospira survival, it seems to be important for the survival of attenuated and saprophytic Leptospira in normal human serum. (AU)

• Processo FAPESP: 11/14347-7 - Mecanismos de evasão do sistema complemento por Leptospira spp: Interação com o inibidor de C1 esterase e C4BP.
• Beneficiário: Leandro Carvalho Dantas Breda
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/09972-0 - Avaliação do potencial vacinal e caracterização bioquímica de termolisina de Leptospira interrogans
• Beneficiário: Lourdes Isaac
• Modalidade de apoio: Auxílio à Pesquisa - Regular