Hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves on Streptococcus mutans biofilm and tooth demineralization

Autor(es)

Pires, Juliana Goncalves ^[1] ; Zabini, Sara Salustiano ^[1] ; Braga, Aline Silva ^[1] ; Fabris, Rita de Cassia ^[1] ; de Andrade, Flaviana Bombarda ^[2] ; de Oliveira, Rodrigo Cardoso ^[1] ; Magalhaes, Ana Carolina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Al Octavio Pinheiro Brisolla 9-75, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 91, p. 17-22, JUL 2018.
• Citações Web of Science: 5

Resumo

Objectives: This study evaluated the effect of the hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (alone or combined) on the viability of Streptococcus mutans biofilm and on the prevention of enamel demineralization. Methods: Strain of S. mutans (ATCC 21175) was reactivated in BHI broth. Minimum inhibitory concentration, minimum bactericidal concentration, minimum inhibition biofilm concentration and minimum eradication biofilm concentration were determined in order to choose the concentrations to be tested under biofilm model. S. mutans biofilm (5 x 10(5) CFU/ml) was produced on bovine enamel, using McBain saliva under 0.2% sucrose exposure, for 3 days. The biofilm was daily treated with the extracts for 1 min. The biofilm viability was tested by fluorescence and the enamel demineralization was measured using TMR. Results: Myracrodruon urundeuva All. (Isolated or combined) at the concentrationsc >= 0.625 mg/ml was able to reduce bacteria viability, while Qualea Grandflora Mart. alone had antimicrobial effect at 5 mg/ml only (p < 0.05). On the other hand, none of the extracts were able to reduce enamel demineralization. Conclusions: The hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (isolated or combined) have antimicrobial action; however, they do not prevent enamel caries under S. mutans biofilm model. (AU)

• Processo FAPESP: 17/00556-0 - Avaliação do efeito antimicrobiano de agentes comerciais e alternativos sobre biofilme microcosmo e sobre a prevenção da desmineralização dentária
• Beneficiário: Ana Carolina Magalhães
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/20212-0 - Avaliação dos extratos hidroalcoólicos das folhas de Myracrodruon urundeuva all. e Qualea Grandiflora Mart. sobre a viabilidade de biofilme de Streptococcus mutans e na prevenção da desmineralização do esmalte in vitro
• Beneficiário: Sara Salustiano Zabini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica