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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects

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Autor(es):
Vendruscolo, Cynthia do Prado [1] ; Moreira, Juliana Junqueira [1] ; Torquato Seidel, Sarah Raphaela [1] ; Fulber, Joice [1] ; Neuenschwander, Henrique Macedo [1] ; Bonagura, Giancarlo [2] ; Agreste, Fernanda Rodrigues [1] ; Arantes Baccarin, Raquel Yvonne [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Clin Med, Sao Paulo, SP - Brazil
[2] Anhembi Morumbi Univ, Dept Large Anim Clin, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 13, n. 5 MAY 29 2018.
Citações Web of Science: 1
Resumo

Objective The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E-2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid. Methods The effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 mu g/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed. Results All joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P<0.05), detected by the lameness measurement system, probably because of the higher ozone concentration. All groups exhibited increased ultrasonography scores on day 14 (P<0.05). Groups A and B exhibited increased proteins concentrations on day 21 (P<0.05). There was no change in hyaluronic acid concentration or the percentage of high-molecular weight hyaluronic acid throughout the experiment. Chondroitin sulfate concentrations decreased in group B, and did not change in group A and C, indicating that neither treatment provoked extracellular matrix catabolism. Cytokine and eicosanoid concentrations were not significantly changed. Conclusions The ozonetherapy did not cause significant inflammation process or cartilage degradation, therefore, ozonetherapy is safe at both evaluated doses. (AU)

Processo FAPESP: 15/04006-9 - Avaliação do efeito inflamatório e oxidante do ozônio medicinal em articulações sinoviais em equinos hígidos
Beneficiário:Cynthia Do Prado Vendruscolo
Modalidade de apoio: Bolsas no Brasil - Mestrado