Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production

Autor(es):Mostrar menos -

Martins, Paula ^[1] ; Machado, Daisy ^[2] ; Theizen, Thais Holtz ^[2] ; Oliveira Guarnieri, Joao Paulo ^[1] ; Bernardes, Bruno Gaia ^[1] ; Gomide, Gabriel Piccirillo ^[1] ; Finzi Corat, Marcus Alexandre ^[3] ; Abbehausen, Camilla ^[4] ; Proenca Modena, Jose Luiz ^[5] ; Odir Rodrigues Melo, Carlos Fernando ^[6] ; Morishita, Karen Noda ^[6] ; Catharino, Rodrigo Ramos ^[6] ; Arns, Clarice Weis ^[5] ; Lancellotti, Marcelo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Fac Pharmaceut Sci FCF, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Biochem & Tissue Biol Dept, Biotechnol Lab, LABIOTEC, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Multidisciplinary Ctr Biol Res, Campinas, SP - Brazil

^[4] Univ Campinas UNICAMP, Inst Chem, Inorgan Dept, Campinas, SP - Brazil

^[5] Univ Campinas UNICAMP, Inst Biol, Genet Mol Biol & Bioagents Dept, Campinas, SP - Brazil

^[6] Univ Campinas UNICAMP, Fac Pharmaceut Sci FCF, INNOVARE Biomarkers Lab, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, MAY 29 2018.
• Citações Web of Science: 4

Resumo

The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1: 160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial tumor cell model (M059J). This research shows efficient strategies without recombinant technology or DNA vaccines. (AU)

• Processo FAPESP: 16/17066-2 - Metabolômica e lipidômica do Zika vírus, do mosquito ao paciente
• Beneficiário: Carlos Fernando Odir Rodrigues Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/12719-0 - Combate à resitência bacteriana: complexos metálicos e a inibição de metalo-beta-lactamses
• Beneficiário: Camilla Abbehausen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Temático