Emergent Properties in Complex Synthetic Bacterial Promoters

Autor(es)

Oliveira Monteiro, Lummy Maria ^[1] ; Arruda, Leticia Magalhaes ^[1] ; Silva-Rocha, Rafael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Syst & Synthet Biol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS SYNTHETIC BIOLOGY; v. 7, n. 2, p. 602-612, FEB 2018.
• Citações Web of Science: 3

Resumo

Regulation of gene expression in bacteria results from the interplay between hundreds of transcriptional factors (TFs) at target promoters. However, how the arrangement of binding sites for TFs generates the regulatory logic of promoters is not well-known. Here, we generated and fully characterized a library of synthetic complex promoters for the global regulators, CRP and IHF, in Escherichia coli, which are formed by a weak -35/-10 consensus sequence preceded by four combinatorial binding sites for these two TFs. Using this approach, we found that while cis-elements for CRP preferentially activate promoters when located immediately upstream of the promoter consensus, binding sites for IHF mainly function as ``UP{''} elements and stimulate transcription in several different architectures in the absence of this protein. However, the combination of CRP- and IHF-binding sites resulted in emergent properties in these complex promoters, where the activity of combinatorial promoters cannot be predicted from the individual behavior of its components. Taken together, the results presented here add to the information on architecture-logic of complex promoters in bacteria. (AU)

• Processo FAPESP: 16/19179-9 - Desvendando as relações arquitetura/função de promotores bacterianos complexos utilizando abordagens de biologia sintética
• Beneficiário: Lummy Maria Oliveira Monteiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/22921-8 - Abordagens de biologia sintética para decifrar os mecanismos de integração de sinais em promotores bacterianos complexos
• Beneficiário: Rafael Silva Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores