Virulence genes, capsular and plasmid types of multidrug-resistant CTXM(-2,-8,-15) and KPC-2-producing Klebsiella pneumoniae isolates from four major hospitals in Brazil

Autor(es)

Andrade, Leonardo Neves ^[1] ; Novais, Angela ^[2] ; Marcato Stegani, Lenita Maria ^[1] ; Ferreira, Joseane Cristina ^[1] ; Rodrigues, Carla ^[2] ; Costa Darini, Ana Lucia ^[1] ; Peixe, Luisa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCFRP, Ribeirao Preto, SP - Brazil

^[2] Univ Porto, Fac Farm, Lab Microbiol, UCIBIO REQUIMTE, Porto - Portugal

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 91, n. 2, p. 164-168, JUN 2018.
• Citações Web of Science: 2

Resumo

We performed a single-month snapshot study of the population diversity of multidrug resistant (MDR) Klebsiella pneumoniae isolates producing carbapenemases and/or extended-spectrum beta-lactamases from four major hospitals in Brazil. Isolates produced diverse ESBL (CTX-M-2, -8, -15, SHV-2), KPC-2 or both (CTX-M-2 and KPC-2), linked to specific genetic backgrounds and plasmids from a few families (IncR, IncFlIk, IncL/M) that were shared among clonal lineages within and between hospitals. A high clonal diversity was identified, among isolates from the same ST (ST11, ST15, ST101 or ST340). Diverse capsular types (n=13 K-types) were identified, most of which linked to specific ST (ST11 and K27 or K64, ST101 and K17, ST340 and KL151, ST15 and K24 or ST17 and KL112). Isolates shared a common set of virulence genes (ureA, fimH, uge, wabG, mrkD, entB) and occasionally ybtS (42%) and kfuBC (18%). Our data suggest intra- and inter-hospital spread of common genetic structures and international MDR K. pneumoniae clones. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/14740-3 - Investigação molecular de linhagens de Klebsiella pneumoniae e plasmídeos carreando genes codificadores de beta-lactamases
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/08892-2 - Investigação molecular de linhagens de Klebsiella pneumoniae e plasmídeos carreando genes codificadores de beta-lactamases
• Beneficiário: Leonardo Neves de Andrade
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/26188-6 - Fatores de virulência e diversidade intraclonal de isolados clínicos de Klebsiella pneumoniae e Escherichia coli produtoras de ESBL e carbapenemases
• Beneficiário: Leonardo Neves de Andrade
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado