Green tea prevents vascular disturbs and attenuates periodontal breakdown in long-term hyperglycaemia in T1D rats

Autor(es)

Catanzaro, Daniela Pereira ^[1] ; Mena Laura, Ever Elias ^[1] ; Cestari, Tania Mary ^[1] ; Nunes Arantes, Ricardo Vinicius ^[1] ; Garlet, Gustavo Pompermaier ^[1] ; Taga, Rumio ^[1] ; Assis, Gerson Francisco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 45, n. 5, p. 557-569, MAY 2018.
• Citações Web of Science: 1

Resumo

Aim: The effects of green tea on the modulation of vascularization during the progression of spontaneous periodontitis in long-term hyperglycaemia in streptozotocin-induced type 1 diabetic (T1D) rats were evaluated. Materials and Methods: Wistar rats normoglycaemic (NG) and T1D were divided into two control groups, which received water (NG-W and T1D-W) and two experimental groups that received green tea (NG-GT and T1D-GT). Periodontal structures were evaluated by microtomographic and histological analyses. Number of immunostained cells for VEGF (NcVEGF+/mm(2)) and CD31 (NcCD31+/mm(2)), as well microvessel density (MVD) in the periodontal ligament (PDL) were evaluated. Results: Long-term hyperglycaemia in T1D-W rats induced vascular alterations in PDL with a reduction of 36% in MVD, a decrease of 33% in NcCD31+/mm(2) and an increase of 53% in NcVEGF+/mm(2). Concomitantly, a severe degree of periodontitis with higher reduction in bone volume and periodontal bone level was observed. In T1D-GT, green tea maintained the MVD, NcCD31+/mm(2) and NcVEGF+/mm(2) in the PDL similar to normoglycaemic groups. Clinically, in T1D-GT rats, green tea reduced dental plaque accumulation and the degree of periodontitis when compared to T1D-W. Conclusion: Daily green tea consumption has a therapeutic effect on the diabetic vascular disorder in PDL and the progression of periodontitis in long-term hyperglycaemia in T1D rats. (AU)

• Processo FAPESP: 12/00680-9 - Análise imunoistoquímica da proteína VEGF e a densidade microvascular no periodonto de ratos diabéticos submetidos ao tratamento com chá verde
• Beneficiário: Daniela Santos Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica