Genotypic Characterization of Arcobacter spp. Isolated from Chicken Meat in Brazil

Autor(es)

Xavier de Oliveira, Maria Gabriela ^[1] ; de Moura Gomes, Vasco Tulio ^[1] ; Vieira Cunha, Marcos Paulo ^[1] ; Moreno, Luisa Zanolli ^[1] ; Moreno, Andrea Micke ^[1] ; Knobl, Terezinha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOODBORNE PATHOGENS AND DISEASE; v. 15, n. 5, p. 293-299, MAY 2018.
• Citações Web of Science: 1

Resumo

Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii are Gram-negative pathogenic microorganisms that cause watery diarrhea and septicemia in humans. The aims of this study were to detect the presence of Arcobacter spp. in chicken meat from butcher shops in SAo Paulo (Brazil) and to verify their virulence genes and genotypic profiles. A total of 300 chicken cuts were analyzed. The results show the presence of Arcobacter spp. in 18.3% of samples, which were identified as A. butzleri (63.6%) and A. cryaerophilus (36.3%). All strains were positive for the virulence genes ciaB and mviN, followed by cj1349 (98%), pldA (94.4%), cadF (72.7%), tlyA (92.7%), hecA (49%), irgA (47.2%), and hecB (34.5%). These strains were subjected to single-enzyme amplified fragment length polymorphism. Nineteen genotypic profiles were obtained for A. butzleri, and 17 for A. cryaerophilus. These results confirm the presence of virulent strains of A. butzleri and A. cryaerophilus in the chicken meat in Brazil. The presence of potentially virulent strains of Arcobacter highlights a possible public health risk, particularly with respect to ingestion of undercooked foods and cross-contamination from uncooked foods during food preparation and contaminated utensils. (AU)

• Processo FAPESP: 14/07837-6 - Análise filogenética e perfil de susceptibilidade antimicrobiana de cepas de Arcobacter spp. isoladas de carnes de aves e suínos
• Beneficiário: Terezinha Knöbl
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/06584-7 - Caracterização genotípica de Arcobacter spp. isolados de carnes de aves comercializada no município de São Paulo-SP
• Beneficiário: Maria Gabriela Xavier de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado