Development and validation of a sensitive LC-MS/MS method to analyze NBOMes in dried blood spots: evaluation of long-term stability

Autor(es)

da Cunha, Kelly Francisco ^[1] ; Eberlin, Marcos Nogueira ^[2] ; Costa, Jose Luiz ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Forensic Toxicology; v. 36, n. 1, p. 113-121, JAN 2018.
• Citações Web of Science: 2

Resumo

Purpose We evaluated the use of dried blood spots (DBS) to determine seven NBOMes by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and also evaluated the stability of these compounds in this dried matrix. Methods An LC-MS/MS method was developed and fully validated to quantify seven NBOMes (25C-, 25H-, 25I-, 25B-, 25G-, 25D-and 25E-NBOMe) in DBS samples. The extraction procedure was optimized using mixture design experiment. Stability study was performed in two different concentrations over 180 days at three different storage temperatures. Results Good linearity, and limits of detection and quantitation of 0.05 and 0.1 ng/mL, respectively, were obtained. The interday imprecision (n = 15) and bias (n = 15) were not higher than 11.4 and 10.3%, respectively, and no carryover was observed. All analytes remained stable in DBS at -20, 4 degrees C and even at room temperatures for 180 days, except 25B-NBOMe and 25I-NBOMe which experienced degradation (22 and 21%, respectively) of the initial concentration at room temperature after 180 days of study. The method was applied to a DBS of an authentic postmortem blood from an NBOMe user, and it was found to be reliable with good selectivity and specificity. Conclusions DBS has been found to allow reliable, sensitive, accurate and robust detection and quantification of seven NBOMes via LC-MS/MS. Also, DBS provided great stability to most of the compounds at room temperature, and no degradation was observed for DBS kept at 4 and -20 degrees C. This is the first trial to analyze NBOMes in DBS samples to our knowledge. (AU)

• Processo FAPESP: 15/10650-8 - Estudo da estabilidade de fármacos, drogas de abuso e praguicidas em amostras de sangue seco em papel, e sua aplicação na toxicologia forense
• Beneficiário: José Luiz da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular