Neonatal- maternal separation primes zymogenic cells in the rat gastric mucosa through glucocorticoid receptor activity

Autor(es)

Ogias, Daniela ^[1] ; Rattes, Isadora C. ^[1] ; Hosoya, Larissa Y. M. ^[1] ; Zulian, Juliana G. ^[1] ; Yan, Chao Yun Irene ^[1] ; Gama, Patricia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, JUN 29 2018.
• Citações Web of Science: 1

Resumo

Neonatal-Maternal Separation (NMS) deprives mammals from breastfeeding and maternal care, influencing growth during suckling-weaning transition. In the gastric mucosa, Mist1 (encoded by Bhlha15 gene) and moesin organize the secretory apparatus for pepsinogen C in zymogenic cells. Our current hypothesis was that NMS would change corticosterone activity through receptors (GR), which would modify molecules involved in zymogenic cell differentiation in rats. We found that NMS increased corticosterone levels from 18 days onwards, as GR decreased in the gastric mucosa. However, as nuclear GR was detected, we investigated receptor binding to responsive elements (GRE) and observed an augment in NMS groups. Next, we demonstrated that NMS increased zymogenic population (18 and and 30 days), and targeted Mist1 and moesin. Finally, we searched for evolutionarily conserved sequences that contained GRE in genes involved in pepsinogen C secretion, and found that the genomic regions of Bhlha15 and PgC contained sites highly likely to be responsive to glucocorticoids. We suggest that NMS triggers GR-GRE to enhance the expression and to prime genes that organize cellular architecture in zymogenic population for PgC function. As pepsinogen C-pepsin is essential for digestion, disturbance of parenting through NMS might alter functions of gastric mucosa in a permanent manner. (AU)

• Processo FAPESP: 12/10948-9 - Impacto da separação materno- neonatal sobre o desenvolvimento da mucosa gástrica em filhotes e ratos jovens: Papel da corticosterona e do receptor GR.
• Beneficiário: Daniela Ogias
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/17415-3 - Desenvolvimento pós-natal da mucosa gástrica: papel da interação entre alimento, hormônios e fatores de crescimento
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21449-9 - Expressão gênica, diferenciação celular e funções gástricas: distúrbios alimentares durante o desenvolvimento pós-natal podem alterar o programa de crescimento em ratos?
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/03076-0 - Diferenciação celular no epitélio gástrico de ratos: avaliação da ação da corticosterona
• Beneficiário: Juliana Guimarães Zulian
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/18459-0 - Diferenciação celular na mucosa gástrica de ratos: papel do padrão alimentar durante o desenvolvimento pós-natal
• Beneficiário: Larissa Yukari Massarenti Hosoya
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica